Human oocyte and embryo vitrification freezing and thawing device and application method thereof

Abstract

The invention provides a human oocyte and embryo vitrification freezing and thawing device and an application method thereof. The lower surface of a rotary vibration tray is fixedly connected with anoutput shaft of a first motor; a liquid outlet pipe penetrates through a through hole in a sliding manner and is fixed on an extending clamp through a clamping assembly; a liquid outlet is formed at the bottom end of the liquid outlet pipe; the liquid outlet is located above an opening of the open groove; the top end of the liquid outlet pipe is internally connected with a driving part which can move vertically along the liquid outlet pipe and is used for sucking or injecting the liquid. According to the human oocyte and embryo vitrification freezing and thawing device provided by the invention, oocytes / embryos are located in an environment with continuous gradient changes of concentration of a freezing solution or a thawing solution in a process of vitrification freezing and thawing in vitro due to a common effect of the liquid outlet pipe and the rotary vibration tray, and operation of repeatedly sucking, blowing, transferring, stirring the embryos and the like of experimenters is avoided; influences of an in vitro environment and man-made operation on irritable stimulation of the embryos are effectively reduced; long-term development of the embryos and the safety of descendantsare facilitated; meanwhile, the working strength of the experimenters is also alleviated.

Description

technical field [0001] The invention belongs to the field of medical equipment, and in particular relates to a human oocyte and embryo vitrification freezing-thawing device and an application method thereof. Background technique [0002] With the liberalization of the country's comprehensive "two-child" policy, there are about 90 million couples in China who meet the two-child standard, of which 60 million are women over the age of 35. Many of these elderly women need assisted reproduction if they want to conceive (test-tube baby) technology. However, due to the superimposed influence of factors such as environmental pollution, high work and life pressure, increasing age, and unhealthy lifestyles, according to the "China Infertility Research Report" jointly issued by the China Population Association and the National Family Planning Commission, China's infertility The incidence of infertility is 12.5%-15%, and the number of patients exceeds 40 million, which means that 1 out...

AI Technical Summary

Problems solved by technology

However, it has only been 20 years since the first successful delivery of human embryos after vitrification and thawing (completed by Mukaida in 1998), and there is still a lack of large-scale data on the safety of offspring produced after vitrification and thawing support

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