2,3',4'-trihydroxy-5-methoxychalcone and extraction method and application thereof

A technology of methoxychalcone and extraction method, which is applied in the field of plant extraction, can solve the problems of lack of research reports on the chemical components and anti-tumor activity of long-sequence chalcones, and achieve the effect of low cost, wide source of raw materials, and simple steps

Pending Publication Date: 2019-03-26
BINZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction and separation of plant active ingredients in my country is relatively late. At present, more than 3,000 kinds of plants (accounting for about 10% of the total number of plants in my country) have been studied on active ingredients, and le

Method used

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  • 2,3',4'-trihydroxy-5-methoxychalcone and extraction method and application thereof
  • 2,3',4'-trihydroxy-5-methoxychalcone and extraction method and application thereof
  • 2,3',4'-trihydroxy-5-methoxychalcone and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] (1) Crush the dried leaves of Vitex longiflora, pass through a 100-mesh sieve, extract three times with 95% (g / g) ethanol at room temperature, use reflux method to extract, and use 10 times (g / ml) in the raw material for the first time The ethanol was extracted for 2 hours, the second time was extracted with ethanol 8 times that of the raw material for 1.5 hours, the third time was extracted with ethanol 6 times that of the raw material for 1 hour, and then the ethanol was recovered until there was no alcohol smell to obtain a liquid extract.

[0062] (2) Disperse 600 g of the extracted liquid extract in 2200 mL of water, sequentially perform systematic solvent extraction with petroleum ether and chloroform, and concentrate the extracts respectively to obtain 32.50 g of petroleum ether extract and 67.36 g of chloroform extract.

[0063] (3) Get 50g of chloroform part extractum, carry out silica gel column chromatographic separation, mix 50g of silica gel in dry method, p...

Embodiment 2

[0067] Other conditions are identical with embodiment 1, only change the ethanol extraction condition in the step (1): the first time with 10.5 times (g / ml) the ethanol extraction 2.5 hours of raw material, the second time with 8.5 times of raw material ethanol Extract for 2 hours, and use 6.5 times of raw material ethanol for the third time to extract for 1 hour.

[0068] The structure was identified according to the method in Example 1, and the identification result was consistent with Example 1, and the obtained product was 2,3',4'-trihydroxy-5-methoxychalcone.

Embodiment 3

[0070] The anticancer activity of the product obtained in Example 1 is tested, and the steps are as follows:

[0071] Experimental cell lines: cervical cancer Hela cells, liver cancer HepG2 cells;

[0072] Test drugs and reagents: DMEM high-glucose medium, fetal bovine serum (FBS), PBS, 0.25% trypsin-EDTA digestion solution, penicillin, streptomycin, CCK-8 kit, 2,3' prepared in Example 1 ,4'-trihydroxy-5-methoxychalcone;

[0073] laboratory apparatus:

[0074] RE-2000A rotary evaporator (Shanghai Yarong Biochemical Instrument Factory), ZF7 three-purpose ultraviolet analyzer (Gongyi Yuhua Instrument Co., Ltd.), -80°C refrigerator (Haier), ultra-clean bench, CO 2 Incubator, microplate reader. AL104 electronic balance (Mettler-Toledo Instrument Co., Ltd.), B-220 constant temperature water bath (Shanghai Yarong Biochemical Instrument Factory), DZF26021 vacuum drying oven (Shanghai Precision Experimental Equipment Co., Ltd.).

[0075] Experimental steps:

[0076]Take out the c...

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Abstract

The present invention provides a compound named 2,3',4'-trihydroxy-5-methoxychalcone, and an extraction method and application thereof, and belongs to the field of plant extraction. The 2,3',4'-trihydroxy-5-methoxychalcone is a novel compound with excellent antitumor activity, and has broad application prospects in preparing anticancer drugs. According to the extraction method of the 2,3',4'-trihydroxy-5-methoxychalcone, the 2,3',4'-trihydroxy-5-methoxychalcone is prepared through ethanol extraction, petroleum ether and chloroform extraction and two-time silica gel column chromatography isolation with leaves of vitex peduncularis as raw materials. The extraction method provided by the invention has the advantages that the steps are simple, the cost is low, the source of the raw materials is wide, and industrial production is easy to implement.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to 2,3',4'-trihydroxy-5-methoxychalcone and its extraction method and application. Background technique [0002] Malignant tumor is a disease that seriously endangers human health and life, and it is also a major problem in the world medical community. In 2008, there were 12.7 million malignant tumor patients and 7.6 million deaths worldwide. The number of deaths from malignant tumors worldwide is more than that of AIDS, malaria and tuberculosis combined. If effective measures are not taken, it is estimated that by 2030, there will be 26 million new cases of malignant tumors every year, and the death toll will reach 17 million. The annual incidence of malignant tumors in my country is about 2 million, and the death toll exceeds 1.4 million, and there is an increasing trend year by year. Overcoming the difficulty of malignant tumors has become an urgent task for medical w...

Claims

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Application Information

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IPC IPC(8): C07C45/78C07C45/79C07C45/80C07C49/84A61P35/00
CPCC07C49/84A61P35/00C07C45/78C07C45/79C07C45/80
Inventor 代现平李孟顺刘明耿枫
Owner BINZHOU MEDICAL COLLEGE
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