Method for increasing yield of major proteins 2 and 3 in royal jelly by liquid chromatography purification

Abstract

The invention provides a method for increasing yield of major proteins 2 and 3 in royal jelly by liquid chromatography purification. The method comprises steps as follows: royal jelly is dissolved inan extraction buffer solution, the mixture is mixed uniformly and centrifuged, a supernatant is taken, saturated ammonium sulfate is added to the supernatant for precipitation, a supernatant is takenafter centrifugation, filtering is performed by a filter membrane, and a sample buffer solution is obtained; the sample buffer solution is replaced with a buffer solution A with pH being 7.0 by an ultrafiltration centrifuge tube; a royal jelly sample is loaded to a pre-assembled weak cationic chromatographic column balanced by the buffer solution A; the column is subjected to gradient elution by amixed solution of the buffer solution A and a buffer solution B, the ratio of the buffer solution B is gradually increased from 0 to 100%, and major proteins 2 and 3 in the royal jelly are separatedand purified in sequence. According to the method for increasing the yield of the major proteins 2 and 3 in the royal jelly by liquid chromatography purification, abundance of the major proteins 2 and3 in the royal jelly is increased, yield of the major proteins 2 and 3 in the royal jelly is increased, repeatability is good, the yield is high, and the major proteins 2 and 3 in the royal jelly canbe produced on a large scale.

Description

technical field [0001] The invention relates to chemical extraction and separation, in particular to a method for increasing the yield of royal jelly major protein 2 and 3 in liquid chromatography purification. Background technique [0002] The protein components of royal jelly are mainly composed of royal jelly major proteins 1, 2 and 3, each of which has unique physiological functions and is beneficial to human health. The traditional methods of purifying royal jelly major proteins 1, 2, and 3 mainly include dialysis, superposition and combination of multiple chromatographic separation methods, etc., which are not only time-consuming, laborious, but also costly and low-yield, which are difficult to meet the needs of biological research and are not It is suitable for large-scale production, and the low abundance is a very important reason. Therefore, it is necessary to develop a simple and rapid sample processing method to increase the corresponding yield of royal jellybum...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of method that improves the yield of royal jelly major protein 2 and 3 in liquid chromatography purification, to overcome the defect that the yield that traditional separation method exists is low

Images