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Preparation method and application of Maryland tobacco leaf fermentation extract

A technology of extract and tobacco leaf, which is applied in the field of preparation of Maryland tobacco leaf fermentation extract, to achieve the effect of increasing extraction rate, increasing yield, and increasing the natural complexity of aroma

Active Publication Date: 2019-03-29
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microbial strains are adsorbed to Maryland tobacco rods, incubated with Maryland tobacco leaf powder in aqueous solution for hydrolysis and fermentation, combined with continuous countercurrent ultrasonic extraction of solvents to prepare Maryland tobacco leaf fermented extracts, and its application in traditional cigarettes and new heat-not-burn cigarettes has not yet been reported.

Method used

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  • Preparation method and application of Maryland tobacco leaf fermentation extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method for immobilized fermented extract of Maryland tobacco leaves, comprising the steps of:

[0028] (1) Pre-treatment of raw materials: Maryland tobacco leaves are pulverized into 40-mesh coarse powder through a pulverizer;

[0029] (2) Activation of microbial strains: add the active solid freeze-dried bacterial powder (food grade, activity 10u / mg) of Saccharomycopsis fibuligera and Enterobacter moriVL4-3 to 10 times the mass In the sterilized 5% glucose solution, place it in a 38°C water bath for activation for 30 minutes to obtain a microbial strain activation solution;

[0030] (3) Preparation of immobilized carrier: wash and dry the immobilized carrier (the tobacco rod of Maryland tobacco) and cut it into small pieces about 1 cm long with scissors, and dry it after autoclaving;

[0031] (4) Immobilization treatment of microbial strains: Weigh the sterilized and dried Maryland tobacco stem fragments in the above step 3) into a 20L barrel, add a cert...

Embodiment 2

[0037] A preparation method for immobilized fermented extract of Maryland tobacco leaves, comprising the steps of:

[0038] (1) Pre-treatment of raw materials: Maryland tobacco leaves are pulverized into 60-mesh coarse powder through a pulverizer;

[0039] (2) Activation of microbial strains: add the active solid freeze-dried bacterial powder (food grade, activity 15u / mg) of Saccharomycopsis fibuligera and Enterobacter moriVL4-3 to 15 times the mass In the sterilized 12% glucose solution, place it in a 45°C water bath for activation for 45 minutes to obtain a microbial strain activation solution;

[0040] (3) Preparation of immobilized carrier: wash and dry the immobilized carrier (the tobacco rod of Maryland tobacco) and cut it into small pieces about 1 cm long with scissors, and dry it after autoclaving;

[0041] (4) Immobilization treatment of microbial strains: Weigh the sterilized and dried Maryland tobacco rod fragments in the above step 3) into a 20L barrel, add a cert...

Embodiment 3

[0047] A preparation method for immobilized fermented extract of Maryland tobacco leaves, comprising the steps of:

[0048] (1) Pre-treatment of raw materials: Maryland tobacco leaves are pulverized to 100 mesh powder by a pulverizer;

[0049] (2) Activation of microbial strains: add the active solid freeze-dried bacterial powder (food grade, activity 20u / mg) of Saccharomycopsis fibuligera and Enterobacter moriVL4-3 to 20 times the mass In the sterilized 20% glucose solution, place it in a 50°C water bath for activation for 60 minutes to obtain a microbial strain activation solution;

[0050] (3) Preparation of immobilized carrier: wash and dry the immobilized carrier (tobacco rod of Maryland tobacco) with scissors and cut it into small pieces with a length of about 1 cm, and dry it after autoclaving;

[0051] (4) Immobilization treatment of microbial strains: Weigh the sterilized and dried Maryland tobacco rod fragments in the above step 3) into a 20L barrel, add a certain a...

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Abstract

The invention provides a preparation method and application of a Maryland tobacco leaf fermentation extract. The extract is prepared by the steps of crushing Maryland tobacco leaves, then immobilizingand adsorbing microbial strains by Maryland tobacco stems, carrying out warm culture hydrolysis fermentation of Maryland tobacco powder, and combining with continuous countercurrent ultrasonic extraction to prepare the Maryland tobacco leaf extract. The extract has strong natural tobacco fragrance, the irritation is reduced, the purification and separation steps are simple, technological conditions are mild, and the extraction rate and the production efficiency are improved; with application of addition of ingredients and spices in traditional cigarettes and novel heating non-combustible cigarettes, the extract has natural tobacco fragrance, adequate vigor and strong sense of penetration, has fruit and milk sweet aroma, and is in harmony with cigarette smoke and consistent style.

Description

technical field [0001] The invention relates to the field of tobacco additives, in particular to a preparation method and application of a Maryland tobacco leaf fermented extract. Background technique [0002] Tobacco additives are additives to improve the physical and chemical properties of tobacco, including flavor essences, humectants, combustion enhancers, anti-mold agents and adsorbents. The production of low-tar cigarettes needs more flavorings to make up for the lack of flavor. The purpose of tobacco product flavoring is to cover up the irritation and miscellaneous gas of natural tobacco, and to enhance or improve its fragrance. Many aroma substances can be identified from tobacco and smoke. The synthesis and application of these spices with the aroma of tobacco itself are being paid more and more attention. Because of its naturalness, safety and natural compound aroma, natural flavors are more catering to consumers' health needs. Among them, tobacco extracts are m...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/20C12P39/00C12P1/04C12P1/02C12N11/02C12R1/645C12R1/01A24B3/12A24B15/20
CPCA24B3/12A24B15/20C12N1/16C12N1/20C12N11/02C12P1/02C12P1/04C12P39/00
Inventor 魏敏宋旭艳李冉罗诚浩何结望
Owner HUBEI CHINA TOBACCO IND