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Adsorption and fermentation preparation method and application of sun-cured yellow tobacco extract

A technology of sun-cured tobacco and extract, which is applied in the field of adsorption and fermentation preparation of sun-cured tobacco extract, which can solve problems such as unfavorable taste, increased irritation, miscellaneous gas and residual feeling

Active Publication Date: 2021-05-18
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional and ordinary tobacco extract preparation methods usually extract flavor substances while containing proteins, tannins, starch, pectin, cellulose, lignin and other macromolecular substances that are unfavorable to cigarette smoking. Also has the drawback of increasing irritation, off-gassing and residual sensations
[0005] At present, there is a patent "a method for preparing molecular distillate of sun-dried tobacco" (application number 201810184517.9), which extracts sun-dried tobacco with ethanol solvent, filters and concentrates it with a ceramic membrane, and then purifies it by molecular distillation, which is then applied in cigarettes; "Preparation method of tobacco sun-cured tobacco extract" (Patent No. ZL201610988163.4) The Maillard reaction liquid obtained by reacting the water extract of sun-cured tobacco with compound amino acids is rectified and applied to new types of tobacco; "Preparation method of substance and essential oil" (Application No. 201110366722.5) uses carbon dioxide supercritical fluid to extract lipids and waxy compounds in air-cured tobacco and other aroma-inducing components, which are used in cigarettes for flavoring; Bioabsorbent materials were used to immobilize active strains, incubate, hydrolyze and ferment sun-cured tobacco leaves, combined with continuous countercurrent ultrasonic extraction technology, obtain the adsorption and fermentation extract of sun-cured tobacco, and apply it in traditional and new heat-not-burn cigarettes. No related reports have been seen yet.

Method used

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  • Adsorption and fermentation preparation method and application of sun-cured yellow tobacco extract
  • Adsorption and fermentation preparation method and application of sun-cured yellow tobacco extract

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A preparation method for adsorption and fermentation of sun-cured tobacco extract, comprising the following steps:

[0028] (1) Raw material pretreatment: sun-cured yellow tobacco leaves are pulverized into 40-mesh powder by a pulverizer;

[0029] (2) Activation of microbial strains: the active solid freeze-dried bacterial powder (food grade, activity of 10u / mg) of Saccharomycopsis fibuligera and Bacillus methylotrophicus (VJ4-1) was added to The mass concentration of 5 times the mass of sterilization treatment is 5% glucose solution, placed in a 35 ° C water bath for activation for 30 minutes, to obtain a microbial strain activation solution, wherein the preservation number of Bacillus methylotrophicus (VJ4-1) is CCTCC NO: 2012004, preserved in the Chinese Type Culture Collection Center of Wuhan University on January 11, 2012;

[0030] (3) Preparation of immobilized carrier: Wash and dry the tobacco rods and beetroots of sun-cured tobacco, cut them into small pieces a...

Embodiment 2

[0039] A preparation method for adsorption and fermentation of sun-cured tobacco extract, comprising the following steps:

[0040] (1) Raw material pretreatment: sun-cured yellow tobacco leaves are pulverized into 80-mesh powder by a pulverizer;

[0041] (2) Activation of microbial strains: the active solid freeze-dried bacterial powder (food grade, activity of 25u / mg) of Saccharomycopsis fibuligera and Bacillus methylotrophicus (VJ4-1) was added to The mass concentration of 8 times the mass of sterilization treatment is 15% glucose solution, placed in a 42 ° C water bath for activation for 40 minutes, to obtain a microbial strain activation solution, wherein the preservation number of Bacillus methylotrophicus (VJ4-1) is CCTCC NO: 2012004, preserved in the Chinese Type Culture Collection Center of Wuhan University on January 11, 2012;

[0042] (3) Preparation of immobilized carrier: wash and dry the tobacco rods and beetroots of sun-cured tobacco, cut them into small pieces ...

Embodiment 3

[0051] A preparation method for adsorption and fermentation of sun-cured tobacco extract, comprising the following steps:

[0052] (1) Raw material pre-treatment: sun-cured yellow tobacco leaves are pulverized into 120-mesh powder by a pulverizer;

[0053] (2) Activation of microbial strains: the active solid freeze-dried bacterial powder (food grade, activity of 30u / mg) of Saccharomycopsis fibuligera and Bacillus methylotrophicus (VJ4-1) was added to The mass concentration of 10 times the mass of sterilization treatment is 30% glucose solution, placed in a 55 ° C water bath for activation for 60 minutes, to obtain a microbial strain activation solution, wherein the preservation number of Bacillus methylotrophicus (VJ4-1) is CCTCC NO: 2012004, preserved in the Chinese Type Culture Collection Center of Wuhan University on January 11, 2012;

[0054] (3) Preparation of immobilized carrier: Wash and dry the tobacco rods and beetroots of sun-cured tobacco, cut them into small piec...

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PUM

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Abstract

The purpose of the present invention is to provide a preparation method and application of adsorption and fermentation of the extract of sun-cured tobacco, using immobilized and adsorbed microbial strains of yellow-cured tobacco stems and beet roots, incubating, hydrolyzing and fermenting the coarse powder of sun-cured tobacco, combined with continuous countercurrent ultrasonic Extraction and preparation of sun-dried tobacco leaf adsorption and fermentation extract, the extract has a strong aroma of natural tobacco, with fruity ester aroma, milk aroma, and roasted aroma, the quality of spices is improved, the purification and separation steps are simple, the process conditions are mild, and the extraction rate and Increased production efficiency; used in traditional cigarettes and new heat-not-burn cigarettes for flavoring and flavoring, with natural tobacco aroma, rich and mellow cigarette aroma, increased concentration, and improved satisfaction, with milk aroma, fruit sour aroma, roasted aroma and The throat feels sweet, the irritation is reduced, and the mouthfeel is improved.

Description

technical field [0001] The invention relates to the field of tobacco additives, in particular to a preparation method and application of an extract of sun-cured tobacco by adsorption and fermentation. Background technique [0002] Tobacco additives are additives to improve the physical and chemical properties of tobacco, including flavor essences, humectants, combustion enhancers, anti-mold agents and adsorbents. The production of low-tar cigarettes needs more flavorings to make up for the lack of flavor. The purpose of tobacco product flavoring is to cover up the irritation and miscellaneous gas of natural tobacco, and to enhance or improve its fragrance. Due to its naturalness, safety and natural compound aroma, natural flavors are more catering to consumers' health needs, but they still have low extraction efficiency, many solvent residues, high impurity content, low purity, complicated purification steps and high cost. Some have obvious characteristic aroma, which will...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/02C12P39/00C12P1/04C12P1/02A24B15/24C12R1/645C12R1/07
CPCA24B15/24C12N11/02C12P1/02C12P1/04C12P39/00Y02P20/133Y02P20/59
Inventor 魏敏宋旭艳潘曦罗诚浩黄龙
Owner HUBEI CHINA TOBACCO IND
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