DNA quantitative analysis method based on microscope images of cells

Abstract

The invention discloses a DNA quantitative analysis method based on microscope images of cells. According to the DNA quantitative analysis method based on the microscope images of cells, an information RAW file obtained directly from a CCD or a CMOS is used as an image for the DNA quantitative analysis, so as to avoid the errors resulting from the increased compensation calculations when calculating an IOD value, to greatly improve the accuracy of DNA quantitative calculation, and to make the calculation result to be more stable when the intensity of the incident light is increased or decreased.

Description

technical field [0001] The invention relates to the technical field of image processing, in particular to a DNA quantitative analysis method based on cell microscope images. Background technique [0002] Cytological examination is the main way for early screening of typical cancers such as cervical cancer and oral cancer. The smear technique used in the early days was popular because of its low price, but its missed diagnosis rate is high. Although the liquid-based smear technique widely used since the 21st century The effect of film production has been improved, but it requires experienced pathologists to directly observe the morphology of cells under a microscope to give diagnostic results, which is inefficient, easily affected by subjectivity, and has poor repeatability. DNA ploidy analysis technology automatically analyzes the nucleus in the cell image, and then quantitatively measures the chromosome or DNA content in the nucleus, and automatically gives the judgment res...

AI Technical Summary

Problems solved by technology

However, this type of method will miss a large number of diploid cells when dealing with densely distributed nuclei and large changes in the gray distribution of the nuclei, and often extracts all the features of each candidate area when identifying them. time-consuming in the case of

[0004] The source steps of the picture in the current DNA quantitative analysis method are as follows: incident light → glass slide → transmitted light → CCD (or CMOS), and finally the CCD (or CMOS) restores the color of the picture to form a color picture or a black picture , when calculating the IOD value in DNA, it is necessary to compensate the RGB value of the picture during the color restoration process of the picture or when calculating the gray value of the picture after grayscale, so accurate IOD cannot be obtained value, so that it will affect the accuracy of DNA quantitative analysis

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