A multi-channel tumor marker parallel detection sensor and its application method

Abstract

A multi-channel tumor marker parallel detection sensor and its use method, related to the field of micro-nano medical detection application technology, providing a plurality of nano-hole sandwich array chips as substrates, each liquid pool of the base near the waste liquid outlet has multiple Flow channels, each flow channel is connected with the liquid pool, each chip is installed in each liquid pool of the base, and bonded to the PDMS cover plate, the nanohole sandwich array chip is placed flat in the middle of each liquid pool of the base, each The solutions on the upper and lower sides of the liquid pool can only be penetrated through the nanopore array, and then the inner surface of the nanopore in the middle layer of the substrate of each channel is modified with tumor marker antibody molecules, and then the detection sample is passed through the channels with a precision syringe pump. Transported to the detection area for parallel detection of multiple tumor markers, the tumor marker molecules are captured by the antibody molecules in the nanopore sandwich array chip, and block current is generated, and the concentration of tumor markers is achieved according to the amplitude of the block current drop. determination.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a multi-channel tumor marker parallel detection sensor and its application method. Background technique [0002] Cancer is the second leading cause of human death, and new cancer cases in my country account for about 1 / 4 of the world's total. The World Health Organization points out that 1 / 3 of cancers can be cured in time through early diagnosis. Tumor markers are substances produced directly by tumor cells or induced by non-tumor cells through tumor cells. Studies have shown that when tumors develop, the concentration of markers is significantly abnormal, indicating the presence of tumors. Tumor marker detection research provides new ideas for non-invasive early diagnosis of cancer. Quantitative detection of tumor markers using micro-nano sensors with special effects such as Coulomb blockade and quantum size effects has also become a major research method for early diagno...

AI Technical Summary

Problems solved by technology

[0004] Studies have shown that the time for a single tumor marker to pass through the nanopore is at the millisecond level, and the time for femtomole-level quantitative detection is more than 100 hours, and the detection efficiency is low

At the same time, there are many forms of single molecules passing through the nanopore, which will generate a variety of blocking current signals and reduce the detection accuracy.

In addition, the tumor markers of a single cancer have diverse characteristics, so realizing the parallel detection of multi-channel tumor markers is a severe challenge for early detection of cancer

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