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Air plant callus and culture method and application thereof

The technology of an air pineapple and a cultivation method, which is applied in the field of plant tissue culture, can solve the problems of unsatisfactory reproduction efficiency, difficulty in obtaining explant materials, etc., and achieve the effects of simple and easy disinfection treatment, easy acquisition, and stable differentiation ability.

Active Publication Date: 2019-04-02
SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, provide a kind of Tillandsia twig callus and its culture method and application, to solve the current technology of obtaining explant materials used for Tillandsia propagation and unsatisfactory reproduction efficiency question

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  • Air plant callus and culture method and application thereof

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Embodiment 1

[0040] This embodiment provides a callus of Tillandsia terrier and its culture method. Described air pineapple callus culture method comprises the steps:

[0041] S11. Disinfection of explants:

[0042] Select the vigorously growing root system of Tillandsia Funkiana, cut off the root tip with a length of 1.0 cm, wash it with sterilized water, soak it for 15 minutes, and transfer it to 0.1% HgCl 2 Disinfect in the solution for 3 minutes, rinse with sterilized water for 5 times;

[0043] S12. Formation of callus:

[0044] Inoculate the root tip after step S11 disinfection on the MS basic medium, inoculate one root tip per bottle, and add 1.0 mg / L of 2,4-dichlorophenoxyacetic acid and 0.5 mg / L of diethylaminoethanol hexanoate Carry out culture treatment, 18-22 days root tip cell division proliferates and forms callus, and callus rate is 80%;

[0045] S13. Subculture proliferation culture:

[0046] The callus formed in the step S12 is transferred to the MS basic medium added...

Embodiment 2

[0048] This embodiment provides a callus of Tillandsia terrier and its culture method. Described air pineapple callus culture method comprises the steps:

[0049] S11. Disinfection of explants:

[0050] Select the vigorously growing root system of Tillandsia Funkiana, cut off the root tip with a length of 0.5 cm, wash it with sterilized water, soak it for 15 min, and transfer it to 0.1% HgCl 2 Disinfect in the solution for 4 minutes, rinse with sterilized water for 5 times;

[0051] S12. Formation of callus:

[0052] Inoculate the root tip after step S11 disinfection on the MS basic medium, inoculate one root tip per bottle, and add 1.2 mg / L of 2,4-dichlorophenoxyacetic acid and 0.5 mg / L of diethylaminoethanol hexanoate Carry out culture treatment, 15-20 days root tip cell division proliferates and forms callus, and callus rate is 73%;

[0053] S13. Subculture proliferation culture:

[0054] The callus formed in the step S12 is transferred to the MS basic medium added wit...

Embodiment 3

[0056] This embodiment provides a callus of Tillandsia terrier and its culture method. Described air pineapple callus culture method comprises the steps:

[0057] S11. Disinfection of explants:

[0058] Select the vigorously growing root system of Tillandsia Funkiana, cut off the root tip with a length of 1.0 cm, wash it with sterilized water, soak it for 10 min, and transfer it to 0.1% HgCl 2 Disinfect in the solution for 5 minutes, rinse with sterilized water for 5 times;

[0059] S12. Formation of callus:

[0060] Inoculate the root tip after step S11 disinfection on the MS basic medium, inoculate one root tip per bottle, and add 1.2 mg / L of 2,4-dichlorophenoxyacetic acid and 1.0 mg / L of diethylaminoethanol hexanoate Carry out culture treatment, 15-18 days root tip cell division proliferates and forms callus, and callus rate is 92%;

[0061] S13. Subculture proliferation culture:

[0062] The callus formed in step S12 is transferred to the MS basic medium added with 0....

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Abstract

The invention discloses a culture method of an air plant callus and a quick propagation method of an air plant. The culture method includes the steps of acquiring root tip tissue of the air plant, anddisinfecting the root tip tissue; inoculating the disinfected root tip tissue to an induction medium to carry out induced culture to obtain a callus; transferring the callus to a subculture enrichment medium to carry out subculture enrichment. An explant material used in the air plant callus culture method is easy to attain, and damage to an original plant is nearly zero; the induced culture andsubculture enrichment for the root tip tissue help acquire massive callus which has stable differentiating capacity. The quick propagation method of the air plant allows the air plant to propagate quickly and massively, and the propagated air plant has stable character.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a callus of air pineapple and its culture method and application. Background technique [0002] Air pineapple belongs to bromeliaceae (Bromeliaceae), which is a monocotyledonous plant and a perennial herb. Bromeliaceae has 3 subfamilies, 50 genera, including nearly 550 varieties and 90 varieties. The three subfamilies are Pitcairnioideae, Tillandsioideae and Bromelioideae. Among them, Tillandsia Funkiana is a common one in Tillandsia Funkiana, which is a typical aerial or epiphytic plant. [0003] The main organ for absorbing nutrients and water in air pineapple is the "sunflower-like" scale structure covered on the leaf surface. This structure helps the air pineapple obtain nutrients and moisture directly from the air where pollutants are present. And their degenerated root system only plays a fixed role on the support and does not need soil, so they...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 陈朋杨蕾蕾张苏州李凌飞郎校安
Owner SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION