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Air pineapple callus and its culture method and application

A technology of Tillandsia truncatula and its cultivation method, which is applied in the field of plant tissue culture, can solve problems such as unsatisfactory reproduction efficiency and difficulty in obtaining explant materials, and achieve the effect of simple and easy disinfection, easy acquisition, and stable differentiation ability

Active Publication Date: 2022-03-18
SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, provide a kind of Tillandsia twig callus and its culture method and application, to solve the current technology of obtaining explant materials used for Tillandsia propagation and unsatisfactory reproduction efficiency question

Method used

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  • Air pineapple callus and its culture method and application

Examples

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Embodiment 1

[0040] This embodiment provides a callus of Tillandsia terrier and its culture method. Described air pineapple callus culture method comprises the steps:

[0041] S11. Disinfection of explants:

[0042] Select the vigorously growing root system of Tillandsia Funkiana, cut off the root tip with a length of 1.0 cm, wash it with sterilized water, soak it for 15 minutes, and transfer it to 0.1% HgCl 2 Disinfect in the solution for 3 minutes, rinse with sterilized water for 5 times;

[0043] S12. Formation of callus:

[0044] Inoculate the root tip after step S11 disinfection on the MS basic medium, inoculate one root tip per bottle, and add 1.0 mg / L of 2,4-dichlorophenoxyacetic acid and 0.5 mg / L of diethylaminoethanol hexanoate Carry out culture treatment, 18-22 days root tip cell division proliferates and forms callus, and callus rate is 80%;

[0045] S13. Subculture proliferation culture:

[0046] The callus formed in the step S12 is transferred to the MS basic medium added...

Embodiment 2

[0048] This embodiment provides a callus of Tillandsia terrier and its culture method. Described air pineapple callus culture method comprises the steps:

[0049] S11. Disinfection of explants:

[0050] Select the vigorously growing root system of Tillandsia Funkiana, cut off the root tip with a length of 0.5 cm, wash it with sterilized water, soak it for 15 min, and transfer it to 0.1% HgCl 2 Disinfect in the solution for 4 minutes, rinse with sterilized water for 5 times;

[0051] S12. Formation of callus:

[0052] Inoculate the root tip after step S11 disinfection on the MS basic medium, inoculate one root tip per bottle, and add 1.2 mg / L of 2,4-dichlorophenoxyacetic acid and 0.5 mg / L of diethylaminoethanol hexanoate Carry out culture treatment, 15-20 days root tip cell division proliferates and forms callus, and callus rate is 73%;

[0053] S13. Subculture proliferation culture:

[0054] The callus formed in the step S12 is transferred to the MS basic medium added wit...

Embodiment 3

[0056] This embodiment provides a callus of Tillandsia terrier and its culture method. Described air pineapple callus culture method comprises the steps:

[0057] S11. Disinfection of explants:

[0058] Select the vigorously growing root system of Tillandsia Funkiana, cut off the root tip with a length of 1.0 cm, wash it with sterilized water, soak it for 10 min, and transfer it to 0.1% HgCl 2 Disinfect in the solution for 5 minutes, rinse with sterilized water for 5 times;

[0059] S12. Formation of callus:

[0060] Inoculate the root tip after step S11 disinfection on the MS basic medium, inoculate one root tip per bottle, and add 1.2 mg / L of 2,4-dichlorophenoxyacetic acid and 1.0 mg / L of diethylaminoethanol hexanoate Carry out culture treatment, 15-18 days root tip part cell division proliferates and forms callus, and callus rate is 92%;

[0061] S13. Subculture proliferation culture:

[0062] The callus formed in step S12 is transferred to the MS basic medium added wi...

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Abstract

The invention discloses a method for cultivating callus of Tillandsia pineapple and a method for rapid propagation of Tillandsia pineapple. The method for culturing the callus of Tillandsia pineapple comprises the following steps: obtaining the root tip tissue of Tillandsia pineapple, and performing disinfection on the root tip tissue; inoculating the sterilized root tip tissue into the induction medium Induction culture treatment was carried out to obtain callus tissue; the callus tissue was transferred to subculture medium for subculture proliferation treatment. The explant material of the air pineapple callus culture method of the present invention is easy to obtain, and basically has zero damage to the original plant; through the induction culture treatment and subculture proliferation culture treatment of the root tip tissue, a large amount of callus can be obtained, and has a stable differentiation ability. The rapid propagation method for Tillandsia pineapples of the present invention can realize rapid and large-scale propagation of Tillandsia twigs, and the propagated Tillandsias have stable properties.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a callus of air pineapple and its culture method and application. Background technique [0002] Air pineapple belongs to bromeliaceae (Bromeliaceae), which is a monocotyledonous plant and a perennial herb. Bromeliaceae has 3 subfamilies, 50 genera, including nearly 550 varieties and 90 varieties. The three subfamilies are Pitcairnioideae, Tillandsioideae and Bromelioideae. Among them, Tillandsia Funkiana is a common one in Tillandsia Funkiana, which is a typical aerial or epiphytic plant. [0003] The main organ for absorbing nutrients and water in air pineapple is the "sunflower-like" scale structure covered on the leaf surface. This structure helps the air pineapple obtain nutrients and moisture directly from the air where pollutants are present. And their degenerated root system only plays a fixed role on the support and does not need soil, so they...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 陈朋杨蕾蕾张苏州李凌飞郎校安
Owner SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION