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Application of Pulsatilla saponin b4 in the preparation of anti-inflammatory bowel disease medicine

A Pulsatilla saponin and inflammatory bowel disease technology, applied in the application field of Pulsatilla saponin B4 in the preparation of anti-inflammatory bowel disease drugs, can solve the problems of inequalities, large differences in chemical structures, and different active groups. To achieve the effect of no toxic side effects, safe and reliable toxic side effects

Active Publication Date: 2021-06-22
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN201510678057 discloses the application of Pulsatilla saponin B4 as an immunomodulator in the treatment of acute inflammation drugs, but only discloses its application in the preparation and treatment of acute liver injury, lung injury and kidney injury, and the disease and treatment mechanism of inflammatory bowel disease It is not the same as the above-mentioned types of tissue damage, and cannot be regarded as the same as the above-mentioned types of tissue damage
In addition, CN2018107093170 discloses the application of Pulsatillae saponin B5 in the preparation of anti-inflammatory bowel disease drugs, but the chemical structures of Pulsatillae saponin B4 and Pulsatillae saponin B5 are very different, and the active groups of the two are different. The curative effect of venereal enteropathy can not be obtained after simple inference based on the existing technology

Method used

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  • Application of Pulsatilla saponin b4 in the preparation of anti-inflammatory bowel disease medicine
  • Application of Pulsatilla saponin b4 in the preparation of anti-inflammatory bowel disease medicine
  • Application of Pulsatilla saponin b4 in the preparation of anti-inflammatory bowel disease medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Effect of Pulsatilla saponin B4 on acetic acid-induced ulcerative colitis in rats

[0034] 40 SD rats, male, 180-220g, were randomly divided into five groups, namely normal control group (Normal), model group (Model), positive control group (mesalazine 200mg / kg, Mesalazine), Pulsatilla saponin B4 (5mg / kg Bid) group (B45mg / kg bid) and Pulsatilla saponin B4 (10mg / kg Bid) group (B4 10mg / kg bid).

[0035] Before the experiment, each group of rats was fasted for 48 hours without water, and 0.5% soapy water was inserted through the anus through a silicone tube with a diameter of 1 mm to wash the intestines, and then intraperitoneally administered with pentobarbital sodium 30m / kg after flushing with clear water. Anesthetized, then the groups were treated as follows:

[0036] Normal control group: insert the catheter 8cm through the anus, inject 1mL of normal saline, put the rat’s head down, and hold the anus up for 20 seconds, then inject 2mL of normal saline for w...

Embodiment 2

[0043] Example 2: Effect of Pulsatilla saponin B4 on DSS-induced ulcerative colitis in mice

[0044] 50 BALB / c mice, male, 18-22g, were randomly divided into five groups, namely normal control group, model group, positive control group (mesalazine 400mg / kg), Pulsatilla saponin B4 (10mg / kg Bid) group and Pulsatilla saponin B4 (20mg / kgBid) group. Model group, positive control group (mesalazine 400mg / kg), Pulsatilla saponin B4 (10mg / kg Bid) group and Pulsatilla saponin B4 (20mg / kg Bid) group were given drinking water containing 25g / L DSS for 6 days, and then replaced with Drinking water normally, mice in the normal control group were given normal drinking water. On the first day after modeling, Pulsatilla saponin B4 (10 mg / kg Bid) group and Pulsatilla saponin B4 (20 mg / kg Bid) group were administered intraperitoneally twice a day, and the positive control group was administered intraperitoneally once a day.

[0045] Disease Activity Index (DAI) From the 1st day of modeling to t...

Embodiment 3

[0048] Example 3: Effect of Pulsatilla saponin B4 on TNBS-induced Crohn's disease in rats

[0049] 40 SD rats, male, 180-220g, were randomly divided into six groups, namely normal control group, model group, positive control group (mesalazine 200mg / kg), Pulsatilla saponin B4 (2.5mg / kg Bid) group , Pulsatilla saponin B4 (5mg / kg Bid) group and Pulsatilla saponin B4 (10mg / kg Bid) group.

[0050] Before the experiment, the rats in each group were fasted for 48 hours without water, and 0.5% soapy water was inserted through the anus through a 1 mm diameter silicone tube to wash the colon, and then rinsed with water. Then anesthetize with pentobarbital sodium 30m / kg intraperitoneal administration, then carry out the following treatment to each group:

[0051] Normal control group: insert the catheter 8cm through the anus, inject 1mL of normal saline, and put the rat’s head down, with the anus facing up and hold it on the hand for 2 minutes; model group and other drug groups: insert ...

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Abstract

The invention relates to the application of Pulsatilla saponin B4 in the preparation of anti-inflammatory bowel disease medicine. The invention also discloses an anti-inflammatory bowel disease medicine, which comprises Pulsatilla saponin B4. The invention discloses for the first time the application of Pulsatilla saponin B4 in the preparation of anti-acute inflammatory bowel disease medicine, belongs to pure natural preparation, is safe, reliable and has no toxic and side effects.

Description

technical field [0001] The present invention relates to the application of Pulsatilla saponin B4, in particular to the application of Pulsatilla saponin B4 in the preparation of anti-inflammatory bowel disease medicine. Background technique [0002] Inflammation can usually be divided into two categories according to the course of the disease: acute inflammation and chronic inflammation. Acute inflammation has a sudden onset and a short duration of only a few days to a month. It is characterized by exudative lesions, and the infiltration of inflammatory cells is mainly granulocytes. Chronic inflammation lasts for a long time, ranging from months to years, and is often dominated by proliferative lesions, and its inflammatory cell infiltration is dominated by macrophages and lymphocytes. [0003] Inflammatory bowel disease (IBD) includes ulcerative colitis (UC) and Crohn's disease (CD). The onset of IBD is insidious, with abdominal pain, diarrhea, mucus stool, and bloody sto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/704A61P29/00A61P1/00A61P1/04
CPCA61K31/704A61P1/00A61P1/04A61P29/00
Inventor 刘艳丽张勇康乃馨沈文华许琼明邵平杨世林
Owner SUZHOU UNIV