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Method and application of increasing fat deposition in animal muscles

A fat deposition and muscle technology, which can be used in medical preparations containing active ingredients, pharmaceutical formulations, active ingredients of hydroxyl compounds, etc., and can solve problems such as reducing the economic value of pork.

Active Publication Date: 2021-02-26
ZHEJIANG HUIJIA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, pork with low IMF content tends to have a higher incidence of PSE (Pale, Soft, Exudative) meat, and the occurrence of PSE pork directly affects the meat color, water retention and eating quality, seriously reducing the economic value of pork

Method used

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  • Method and application of increasing fat deposition in animal muscles
  • Method and application of increasing fat deposition in animal muscles
  • Method and application of increasing fat deposition in animal muscles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Establishment of an animal model with high intramuscular fat deposition

[0038]1. Preparation of ZJU-GLM1 preparation: According to the regeneration characteristics of muscle tissue and the biological knowledge of muscle stem cells, a preparation that can induce muscle tissue damage and fat deposition was prepared, and the preparation was named ZJU-GLM1.

[0039] Each 100ml preparation ZJU-GLM1 consists of the following components: 50% glycerol, 0.1mM (mmol / L) betaine, 0.1mM linoleic acid, and the balance is 0.9% sodium chloride aqueous solution.

[0040] That is, in 50ml of glycerin, 0.01mmol of betaine, and 0.01mmol of linoleic acid, 0.9% sodium chloride aqueous solution was added and the volume was adjusted to 100ml.

[0041] 2. Experimental animal anesthesia: Anesthetize mice around 10-12 weeks old with anesthesia reagents. The anesthesia method is: prepare 1% pentobarbital sodium with 0.9% normal saline, and calculate the injection dose after weighing the mice...

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Abstract

The invention discloses a preparation ZJU-GLM1 for inducing muscle tissue damage and fat deposition. Every 100ml of the preparation ZJU-GLM1 consists of the following components: 40-60ml glycerin, 0.1-1mM betaine, 0.1-1mM linoleic acid , and the balance is an aqueous sodium chloride solution with a mass concentration of 0.85 to 0.9%. The present invention also simultaneously provides a method for improving intramuscular fat deposition in animal skeletal muscle, using intramuscular injection of ZJU-GLM1 preparation, the injected muscle is the tibialis anterior muscle (TA) muscle, and the sampling time is 14 to 28 days after injection; Intramuscular fat deposition model. The invention can be used to study the influence of a muscle model with high fat deposition on glucose tolerance and metabolism, and can be extended to increase the fat content in the muscle in pig production.

Description

technical field [0001] The invention relates to a method and application for increasing fat deposition in animal muscles, and belongs to the applied technology in the technical fields of biology and modern agriculture. Background technique [0002] Skeletal muscle is the dynamic part of the motor system. It is mainly composed of muscle cells (muscle fibers) arranged in bundles. Each cell is covered with a fine reticular membrane called endomysium; each muscle bundle is wrapped by the perimysium. On the outside of each muscle, there is a thick layer of connective tissue called epimysium. Muscle fibers are divided into slow-twitch (I) and fast-twitch (IIA, IIB, IIX) according to contraction characteristics; according to metabolic characteristics: oxidative (I, IIA) and glycolytic muscle fibers (IIB, IIx). In normal muscle tissue, there is a small amount of fat deposition, and it is closely related to the type of muscle fiber. Faster twitch fibers contain more mitochondria an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/201A61K31/047A61K31/205
CPCA61K31/047A61K31/201A61K31/205A61K2300/00
Inventor 单体中徐子叶有文静刘嘉琪汪以真
Owner ZHEJIANG HUIJIA BIOTECH CO LTD
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