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Large Stokes orange fluorescent protein lssmko1 and its applications

A fluorescent protein and Stokes technology, applied in the field of bio-optics and molecular imaging, can solve the problems of imaging crosstalk, few types of LSSOFP, and large overlap of emission spectra, so as to improve resolution, depth, sensitivity, and brightness high effect

Active Publication Date: 2022-07-05
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, there are very few types of LSSOFPs available now, and the emission spectra of existing LSSOFPs and LSSRFPs overlap greatly, resulting in serious imaging crosstalk. Therefore, it is necessary to develop a new LSSOFP with a blue-shifted emission spectrum.

Method used

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  • Large Stokes orange fluorescent protein lssmko1 and its applications
  • Large Stokes orange fluorescent protein lssmko1 and its applications
  • Large Stokes orange fluorescent protein lssmko1 and its applications

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Embodiment Construction

[0020] Hereinafter, the inventive concept will be described in more detail with reference to the accompanying drawings. However, the inventive concept may be implemented in various different forms and should not be construed as limited to the technical solutions only shown here. Rather, these embodiments are provided as examples so that this disclosure will be thorough and complete, and will fully convey the inventive concept to those skilled in the art. Thus, experimental procedures, conditions, instruments, etc., which are not necessary for a person of ordinary skill in the art to fully understand the various aspects and features of the present inventive concept, may not be described. If the specific conditions are not indicated in the examples, it is carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used without the manufacturer's indication are conventional products that can be purchased from ...

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Abstract

The present invention provides the large Stokes orange fluorescent protein LSSmKO1 and its application. The large Stokes orange fluorescent protein is a mutated orange fluorescent protein mKOκ. Compared with the amino acid sequence of mKOκ, the large Stokes orange fluorescent protein has a mutation site: V160D.

Description

technical field [0001] The invention relates to the technical field of bio-optics and molecular imaging, in particular to a large Stokes orange fluorescent protein LSSmKO1 and the application of the large Stokes orange fluorescent protein LSSmKO1. Background technique [0002] When using fluorescent proteins for live cell or in vivo imaging, it is often difficult to achieve multi-color simultaneous imaging. If this limitation can be overcome, the detection of multiple biological rapid response events can be achieved, which will greatly promote the development of neurobiology. [0003] In single-photon imaging, multicolor simultaneous imaging can be achieved by exciting multiple fluorescent proteins with different excitation wavelengths of light. However, in multiphoton imaging, the cost of adding a second-stage Ti:Sapphire laser or an optical parametric oscillator is high, and it is difficult to tune them together, so three-color or multi-color simultaneous imaging of fluore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00G01N21/64
CPCG01N21/6486C07K14/00
Inventor 储军郭育奇张楚秋刘丰
Owner SHENZHEN INST OF ADVANCED TECH