Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Human anti-Mullerian hormone assay kit with high sensitivity and wide detection range

A kit and hormone technology, applied in the field of immunodetection, can solve the problems of inaccurate detection results, high linearity values, and poor reagent precision.

Inactive Publication Date: 2019-04-12
BEIJING BEIER BIOENG
View PDF8 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of chromatography are poor precision of reagents, inaccurate detection results, and insufficient high linear high value; the disadvantages of enzyme-linked immunoassay are long detection time, cumbersome operation steps, large system error, and insufficient high linear high value; electrochemiluminescence The disadvantage of this method is that the detection cost is high, the plasma samples anticoagulated with EDTA and sodium citrate cannot be detected, and the linear high value is not high enough

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Human anti-Mullerian hormone assay kit with high sensitivity and wide detection range
  • Human anti-Mullerian hormone assay kit with high sensitivity and wide detection range
  • Human anti-Mullerian hormone assay kit with high sensitivity and wide detection range

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] A human anti-Müllerian hormone assay kit, comprising reagent 1, reagent 2 and human AMH calibrator with different gradient concentrations; wherein,

[0067] Reagent 1 included the following final concentrations of components: 30 mM HEPES buffer (pH 7.2), 1% (w / v) BSA, 1% (w / v) PEG20000, 1% (w / v) Mw Be the dextran of 80000, the Mw of 1.5% (w / v) is the dextran of 40000, the NaCl of 150mM, the TECP of 10mM, the Triton X-100 of 0.1% (w / v), 0.1% (w / v ) ProClin300, 5mM EDTA;

[0068] Reagent 2 includes the components of the following final concentrations: carboxyl latex microspheres (average particle diameter is 309nm, microsphere final concentration is 0.01% (w / v)), 0.005% (w / v ) labeled human AMH monoclonal antibody, carboxyl latex microspheres labeled only with human AMH polyclonal antibody (average particle size is 107nm, final concentration of microspheres is 0.2% (w / v)), 0.045% (w / v) Labeled human AMH polyclonal antibody, 50mM MOPS buffer (pH7.4), 1.5% (w / v) BSA, 0.2%...

Embodiment 2

[0075] A human anti-Müllerian hormone assay kit, comprising reagent 1, reagent 2 and human AMH calibrator with different gradient concentrations; wherein,

[0076] Reagent 1 included the following final concentrations of components: 50 mM MOPS buffer (pH 7.4), 1.5% (w / v) BSA, 3% (w / v) PEG6000, 3% (w / v) Mw 5000 polylysine, 150mM NH 4 Cl, DTT of 5mM, Tween-80 of 0.05% (w / v), ProClin300 of 0.1% (w / v), EDTA of 5mM;

[0077] Reagent 2 includes the following final concentration components: carboxyl latex microspheres labeled with human AMH monoclonal antibody (average particle diameter is 273nm, microsphere final concentration is 0.015% (w / v)), 0.01% (w / v) The labeled human AMH monoclonal antibody, carboxyl latex microspheres labeled with human AMH polyclonal antibody (the average particle diameter is 88nm, the final concentration of microspheres is 0.25% (w / v)), 0.042% (w / v) of the label Human AMH polyclonal antibody, 50mM PBS buffer (pH7.3), 1% (w / v) BSA, 0.3% (w / v) gelatin, 5% ...

Embodiment 3

[0084] A human anti-Müllerian hormone assay kit, comprising reagent 1, reagent 2 and human AMH calibrator with different gradient concentrations; wherein,

[0085] Reagent 1 includes the following final concentrations of components: 50 mM Tris-Cl buffer (pH 8.0), 0.5% (w / v) BSA, 2% (w / v) PEG8000, 0.5% (w / v) The Mw is the polylysine of 500000, the KCl of 100mM, the DTT of 10mM, the Triton X-100 of 0.1% (w / v), the ProClin300 of 0.1% (w / v), the EDTA of 2mM;

[0086] Reagent 2 includes the following final concentration components: carboxyl latex microspheres labeled with human AMH monoclonal antibody (average particle diameter is 240nm, microsphere final concentration is 0.015% (w / v)), 0.012% (w / v) Labeled human AMH monoclonal antibody, carboxyl latex microspheres labeled with human AMH polyclonal antibody (average particle diameter is 107nm, microsphere final concentration is 0.2% (w / v)), 0.038% (w / v) labeling Human AMH polyclonal antibody, 50mM HEPES buffer (pH7.4), 0.5% (w / v) ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The average particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of immunoassay, and in particular to a human anti-Mullerian hormone assay reagent kit with high sensitivity and the wide detection range. The human anti-Mullerian hormone (AMH) assay reagent kit comprises a reagent 1, a reagent 2, and human AMH calibrators of different gradient concentrations, wherein the reagent 2 comprises carboxylate latex microspheres labeledonly with human AMH monoclonal antibodies and carboxylate latex microspheres labeled only with human AMH polyclonal antibodies, and the average particle diameter of the carboxylate latex microsphereslabeled only with the human AMH monoclonal antibodies is greater than that of the carboxylate latex microspheres labeled only with the human AMH polyclonal antibodies. According to the human AMH assay reagent kit, detection of a single sample can be finished within 10 minutes, and indexes such as precision, accuracy and interference resistance are excellent.

Description

technical field [0001] The invention relates to the field of immunoassay, in particular to a human anti-Müllerian hormone assay kit with high sensitivity and wide detection range. Background technique [0002] Anti-Müllerian hormone (AMH) was first discovered clinically in 1953. It is a homodimeric glycoprotein (110KDa N-terminal, 25KDa C-terminal) in the transforming growth factor superfamily TGF-β member, and its gene is located in On p13.2-p13.3 of the short arm of chromosome 19, it can bind to receptor II and exert biological effects. In 1981, Hutson found for the first time that ovarian granulosa cells can also produce AMH, and both men and women can secrete AMH. [0003] In males, AMH is involved in the differentiation and development of the testis, secreted by immature Sertoli cells in the testis, responsible for the decline of the Mullerian duct and the normal development of the male reproductive tract. The serum AMH level rises after birth, and the AMH level is hi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/74G01N33/577
CPCG01N33/577G01N33/74
Inventor 不公告发明人
Owner BEIJING BEIER BIOENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com