Application of Rendan mini-pills in preparing medicine for preventing or treating Huntington's disease
A technique of chorea and dandruff, applied in the directions of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of patients with great harm, cognitive impairment, abnormal behavior, etc.
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Embodiment 1
[0020] The preparation of embodiment one dandelion ethanol extract
[0021] The present invention has no particular limitation on the source of the rendan, and it is prepared by using the known rendan formula and drug standards in the art (such as the preparation method of the rendan recorded in the patent with application number 200910194559.1), or purchased from the market can be.
[0022] In the embodiment of the present invention, the human pill is provided by Shanghai Zhonghua Pharmaceutical Co., Ltd., and the basic information of the drug is as follows:
[0023] Chinese name: Dragon and Tiger Pill
[0024] [Dosage Form] Pills (paste pills)
[0025] [Preparation method] Take borneol and menthol from the above-mentioned medicinal materials, add appropriate amount of 95% ethanol to make a mixed solution; take catechu and add appropriate amount of water, heat to dissolve, filter to obtain catechu solution, add 4.5g sodium benzoate, 180.5 g glutinous rice flour, stir well ...
Embodiment 2
[0030] Example Erdan alcohol extract index components are measured by HPLC
[0031] 1. Sample treatment: Weigh 100mg of the ethanol extract, add 10ml of 50% methanol, weigh it, and ultrasonically treat it for 10 minutes. After cooling at room temperature, weigh it again, make up the lost weight with 50% methanol, filter, Take the filtrate, that is.
[0032] 2. Preparation of standard curve: weigh catechin and epicatechin, prepare mother liquor, dilute step by step with 50% methanol solution and prepare standard working series solutions, the concentrations are 50μg / mL, 100μg / mL, 250μg / mL , 500 μg / mL, 1.0 mg / mL, chromatographic injection of 10 μL, and draw a standard curve with the peak areas of catechin and epicatechin measured respectively for their concentrations.
[0033] 3. Chromatographic conditions and system suitability test use octadecylsilane bonded silica gel as filler, chromatographic column: 250mm*4.6mm*5μm, mobile phase is 2% glacial acetic acid (A)-acetonitrile (...
Embodiment 3
[0040] Therapeutic effect of embodiment Sanrendan alcohol extract on Huntington's disease
[0041] 1. Biomaterials
[0042] (1) Caenorhabditis elegans Caenorhabditis elegans AM141 was purchased from the Caenorhabditis Genetics Center (CGC); it is a transgenic strain based on humanized polyQ nematode without nerve damage, polyQ is fused with YFP and expressed in muscle, and has neuroprotective effect. The test drug can significantly reduce polyQ punctate fluorescence. In this example, the Caenorhabditis elegans AM141 strain was used as a pathological model for screening anti-HD drugs.
[0043] (2) Escherichia coli OP50 (uracil-leakage mutant) was purchased from Caenorhabditis Genetics Center (CGC) and used as food for Caenorhabditis elegans.
[0044] 2. Reagents
[0045] (1) Solid NGM (Nematode Growth Medium) medium composition and production (take 1 liter as an example):
[0046] Element
content
NaCl
3.00g
K 2 HPO 4
2.34g
K H 2 ...
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