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Method for preparing high-vigority Aspergillus niger citrate seeds by full utilization of corn liquefaction residue

A technology for liquefying residue and citric acid, applied in the field of waste recycling and fermentation, can solve the problems of aggravating the burden of environmental pollution, waste of resources, etc., and achieve the effects of improving the activity of bacteria, improving the utilization rate and reducing the production cost.

Active Publication Date: 2022-04-01
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, some studies have been carried out on the utilization of corn residues. The patent application No. 201310003366.X's invention patent "Using compound enzymes to hydrolyze citric acid to ferment corn residues to obtain reducing sugars" discloses compound enzyme preparations (comprising cellulase, Xylanase, mesophilic amylase and glucoamylase) are put into the heated corn dregs slurry, and the enzymatic hydrolysis time is 3-5 hours. After the hydrolyzed slurry is filtered, the filtrate containing reducing sugar is obtained. The disadvantage is that this method only obtains Part of the reducing sugar in the corn dregs is eliminated, and most of the protein and residual sugar in the residue are not efficiently utilized, which not only causes waste of resources, but also increases the burden of environmental pollution

Method used

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  • Method for preparing high-vigority Aspergillus niger citrate seeds by full utilization of corn liquefaction residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The corn flour is liquefied by spraying, and the solid-liquid separation of the plate and frame filter is carried out to obtain the corn liquefaction residue; the liquefaction residue is placed in a linear accelerator device, and the electron beam radiation treatment is performed, and the radiation treatment intensity is 5kGy to obtain the radiation residue; the radiation residue is mixed with water according to 1 : 1 mix evenly, and place in ultrasonic soaking treatment, soaking time is 40min, adjust soaking temperature to 55°C; adjust pH of soaking solution to 4.5, add cellulase 40FPU / g, hydrolyze for 1h, further add protease 80U / g, enzymatically hydrolyze 1h; add compound glucoamylase to the enzymolysis solution for enzymolysis, the compound glucoamylase contains glucoamylase and pullulanase, the amount of compound glucoamylase added is 150U / g, the enzymolysis time is 3h, and the liquefaction residue hydrolyzate is obtained; liquefaction The residue hydrolyzate and wa...

Embodiment 2

[0018] The corn flour is liquefied by spraying, and the solid-liquid separation of the plate and frame filter is used to obtain the corn liquefaction residue; the liquefaction residue is placed in a linear accelerator device, and the electron beam radiation treatment is performed, and the radiation treatment intensity is 10kGy to obtain the radiation residue; the radiation residue is mixed with water according to 1 : 1 mix evenly, and place in ultrasonic soaking treatment, soaking time is 30min, adjust soaking temperature to 55°C; adjust pH of soaking solution to 4.5, add cellulase 60FPU / g, hydrolyze for 1h, further add protease 50U / g, enzymatically hydrolyze 1h; add compound glucoamylase to the enzymolysis solution for enzymolysis, the compound glucoamylase contains glucoamylase and pullulanase, the amount of compound glucoamylase added is 100U / g, the enzymolysis time is 3h, and the liquefaction residue hydrolyzate is obtained; liquefaction The residue hydrolyzate and water we...

Embodiment 3

[0020] The corn flour is liquefied by spraying, and the solid-liquid separation of the plate and frame filter is used to obtain the corn liquefaction residue; the liquefaction residue is placed in a linear accelerator device, and the electron beam radiation treatment is performed, and the radiation treatment intensity is 20kGy to obtain the radiation residue; the radiation residue is mixed with water according to 1 : 1 mix evenly, and place in ultrasonic soaking treatment, soaking time is 20min, adjust soaking temperature to 55°C; adjust pH of soaking solution to 4.5, add cellulase 20FPU / g, hydrolyze for 1h, further add protease 60U / g, enzymatically hydrolyze 1h; add compound glucoamylase to the enzymolysis solution for enzymolysis, the compound glucoamylase contains glucoamylase and pullulanase, the amount of compound glucoamylase added is 200U / g, the enzymolysis time is 2h, and the liquefaction residue hydrolyzate is obtained; liquefaction The residue hydrolyzate and water we...

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Abstract

The invention discloses a method for preparing high-activity Aspergillus niger citrate seeds by fully utilizing corn liquefaction residue, which belongs to the technical field of waste recycling and fermentation. Corn flour is liquefied by spraying and solid-liquid separation to obtain corn liquefaction residue; corn residue is treated with electron beam radiation to obtain radiation residue; radiation residue is mixed with water in a certain proportion, fully soaked in an ultrasonic environment, and cellulase and protease are added in sequence Carrying out hydrolysis; adding compound glucoamylase (including glucoamylase and pullulanase) to the above enzymolysis solution to obtain a hydrolyzate of liquefaction residue; configuring seed culture medium with the hydrolysis solution of liquefaction residue, and cultivating Aspergillus niger citrate seeds. The invention realizes full utilization of corn liquefaction residue, obtains high-vigority Aspergillus niger citrate seeds, improves economic benefits, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of waste recycling and fermentation, and in particular relates to a method for preparing high-vigority citrate Aspergillus niger seeds by fully utilizing corn liquefaction residue. Background technique [0002] Corn is a very important food raw material and plays an important role in the sugar industry and fermentation industry. In the fermentation industry, corn flour needs to undergo a certain liquefaction treatment to degrade the macromolecular starch polymer into small molecular dextrin, glucose, etc. before it can be used by microorganisms. The corn flour liquefaction process adopts secondary injection liquefaction, adding high-temperature α-amylase (injection temperature 120-140°C), and liquefying at 90-95°C for 1-2 hours to obtain corn flour liquefaction liquid, which is filtered through plate and frame Solid-liquid separation to obtain liquefied supernatant and liquefied residue respectively. The li...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/685
CPCC12N1/14
Inventor 王宝石张明霞李林波谭凤玲胡舒婕熊小川岳昌琼吴豪
Owner HENAN INST OF SCI & TECH