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A Near Infrared Fluorescence Sensitization Method for Determination of Glucose

A glucose and near-infrared technology, applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve problems affecting detection results, probe fluorescence quenching, false positive signals, etc., to improve sensitivity and avoid False positive interference, the effect of broadening the linear range

Active Publication Date: 2021-05-14
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the conventional fluorescence method is based on the quantitative detection of the concentration relationship between the degree of fluorescence quenching and the analyte, and in practical applications, the impurity of the sample may cause the quenching of the probe fluorescence, causing false positive signals. final impact on test results

Method used

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  • A Near Infrared Fluorescence Sensitization Method for Determination of Glucose
  • A Near Infrared Fluorescence Sensitization Method for Determination of Glucose
  • A Near Infrared Fluorescence Sensitization Method for Determination of Glucose

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Embodiment 1

[0024] Embodiment 1: A near-infrared fluorescence sensitization method is used to measure glucose, using gold nano-glucose oxidase hybrid material as a near-infrared fluorescent probe, after adding glucose, the near-infrared fluorescence of gold nanoparticles is enhanced, and the degree of enhancement of fluorescence is the same as The concentration of glucose is proportional, so as to realize the detection of glucose.

[0025] (1) Synthesis of gold nanoparticles: weigh 0.0394g of HAuCl 4 ∙3H 2 O and 0.0490 g of N-acetyl-L-cysteine ​​were mixed and dissolved in 5 ml of methanol / glacial acetic acid (volume ratio of 6:1) organic solvent, placed in an ice bath, under stirring Quickly add an ethanol solution containing 0.0775 g of sodium borohydride, keep stirring to control the reaction time for 30 min, then add 10 ml of acetone, and remove the solvent by centrifugation to obtain the crude product of gold nanoparticles. The obtained crude product was dissolved in 1 ml of second...

Embodiment 2

[0030] Embodiment 2: A near-infrared fluorescence sensitization method is used to measure glucose, using gold nano-glucose oxidase hybrid material as a near-infrared fluorescent probe, after adding glucose, the near-infrared fluorescence of gold nanoparticles is enhanced, and the degree of enhancement of fluorescence is the same as The concentration of glucose is proportional, so as to realize the detection of glucose.

[0031] (1) Synthesis of gold nanoparticles: weigh 0.0394g of HAuCl 4 ∙3H 2 O and 0.0572 g of N-acetyl-L-cysteine ​​were mixed and dissolved in 6 ml of methanol / glacial acetic acid (6:1 by volume) organic solvent, placed in an ice bath, under stirring Quickly add an ethanol solution containing 0.0775 g of sodium borohydride, keep stirring to control the reaction time for 40 min, then add 10 ml of acetone, and remove the solvent by centrifugation to obtain the crude product of gold nanoparticles. The obtained crude product was dissolved in 1 ml of secondary wa...

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Abstract

The invention belongs to the technical field of preparation and application of functional materials, and provides a near-infrared fluorescence sensitization method for measuring glucose in order to solve the problems of false positives and serious interference in conventional fluorescence quenching methods. The present invention uses the gold nano-glucose oxidase hybrid material as a near-infrared fluorescent probe. After adding glucose, the near-infrared fluorescence of gold nanoparticles is enhanced, and the degree of fluorescence enhancement is proportional to the concentration of glucose, thereby realizing the detection of glucose. . The detection method is easy to operate and fast in response, and the maximum emission of the fluorescent probe is in the near-infrared region, which can effectively avoid the interference of coexisting substances, especially the autofluorescence and Raman Rayleigh scattering of biological samples. Compared with fluorescence quenching method, it has higher sensitivity and selectivity.

Description

technical field [0001] The invention belongs to the technical field of preparation and application of functional materials, and in particular relates to a near-infrared fluorescence sensitization method for measuring glucose. Background technique [0002] Glucose is an essential nutrient in the basic life activities of organisms. It can directly participate in metabolism and is of great significance to ensure the energy supply of various tissues and organs of the human body and maintain human health. In addition to physiological necessity, glucose has a wide range of applications in food industry, pharmaceutical manufacturing, environmental monitoring and biological process monitoring. [0003] In recent years, people have developed a variety of methods for the detection of glucose content, such as high performance liquid chromatography, optical rotation, spectrophotometry, electrochemical methods and fluorescence methods. However, these methods have certain disadvantages. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/17B22F9/24B22F1/00B82Y30/00
CPCG01N21/1717G01N21/6428B82Y30/00B22F9/24G01N2021/1725B22F1/054
Inventor 董文娟尉江燕弓晓娟梁文婷樊丽董川
Owner SHANXI UNIV
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