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Water body organic pollution toxicity assessment method based on human-mouse hybridoma cell

A human-mouse hybridoma and organic pollution technology, which is applied in the field of toxicity evaluation of organic pollution in water bodies, can solve the problems of not reflecting the potential toxicity hazards of pollutants, and achieve the effects of high degree of automation, good reproducibility, and wide application range

Active Publication Date: 2015-05-20
安徽科博产品检测研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the principles of these technologies to detect water pollution are all based on the physical or chemical characteristics of pollutants, which cannot reflect the potential toxic hazards of pollutants.

Method used

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  • Water body organic pollution toxicity assessment method based on human-mouse hybridoma cell
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  • Water body organic pollution toxicity assessment method based on human-mouse hybridoma cell

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Experimental program
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Effect test

Embodiment 1

[0033] The effect of the enrichment of water samples in the Jialu River flood season on the cell survival rate was detected.

[0034] This embodiment includes the following steps:

[0035] (1) Extraction of organic matter in water samples;

[0036] Such as figure 1 As shown, the device for extracting water samples in this embodiment includes a raw water container 1, an acetone container 2, a nitrogen cylinder 3, an extraction column 4, a waste liquid container 5, a rich liquid container 6, a first valve 7, a second valve 8, The third valve 9, the fourth valve 10 and the three-way valve 11, the raw water container 1 communicates with the first valve 7, the nitrogen cylinder 3 is respectively connected with the second valve 8 and the fourth valve 10, and the acetone container 2 is connected with the third valve 9 connected, the waste liquid container 5 and the enriched liquid container 6 are respectively connected to the three-way valve 11, the inlet of the extraction column 4...

Embodiment 2

[0053] To detect the effect of water sample enrichment in dry season of Jialu River on cell viability.

[0054] Method is identical with embodiment 1. In order to compare the cytotoxicity of the water sample enrichment between the wet season and the dry season, five water samples collected in March 2010 were also studied for cell colony formation ability. image 3 Show, when dry season, for the water sample enrichment of 50ml / ml dose group, S M-1 Water sample enrichment treatment A L After 24 hours, the viability of the cells was 0 ( image 3 A); S M-2 Water sample enrichment treatment A L After 24 hours, the viability of the cells decreased significantly to 19.74±10.49% (p image 3 B), down about 80%. However, the same water sample enrichment of the 50ml / ml dose group, S N-2 Water sample enrichment treatment A L After 24 hours, there was no significant effect on the clone survival of cells ( image 3 D); S N-1 and S N-3 Water sample enrichment treatment A L After 24...

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Abstract

The invention discloses a water body organic pollution toxicity assessment method based on a human-mouse hybridoma cell. The method comprises the steps of: extracting organic matters in a water sample; contaminating the cell through utilizing the organic matters of the water sample; clonally culturing; and quantizing the toxicity amount. The method can be utilized for the toxicity assessment of different type water body organic pollutions based on living organisms, so that the defects of detecting and assessing the water pollution on the basis of water body physical and chemical indexes are overcome; the method does not have specificity on organic poison types and has a wide application scope; a petri dish after wide ion beam radiation modification is contributed to cell adherence to the petri dish, the growth and survival of the cell are benefited, and the accuracy of the assessment method is improved; compared with a crystal violet stain and a giemsa stain, a methylene blue stain is low in cost and small in cell toxicity, has good economic and environmental benefits and is thus selected to stain for cell cloning.

Description

technical field [0001] The invention relates to a method for identifying and evaluating water pollution, in particular to a method for evaluating the toxicity of water body organic pollution based on human-mouse hybridoma cells. Background technique [0002] At present, the situation of water pollution in the world is already very serious. It is said that more than 420 billion cubic meters of sewage are discharged into rivers, lakes and seas in the world every year, polluting 5.5 trillion cubic meters of fresh water, accounting for more than 14% of global runoff. Moreover, it is showing a deteriorating trend. Because the water source is polluted by heavy metal ions and toxic organic matter, it seriously threatens human health. These substances can cause deformities, cancer, and organ lesions in people. At present, my country's research in the field of environment mostly focuses on the detection methods of toxic chemical substances in the environment and the development of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/18
Inventor 吴李君王晓飞王红强蔡冬清陈少鹏许安
Owner 安徽科博产品检测研究院有限公司
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