δ integration and secretion of industrial strains of Saccharomyces cerevisiae expressing cellulase and its application

A technology of cellulase and Saccharomyces cerevisiae, which is applied in the field of bioengineering, can solve the problems of low enzyme diffusion rate, achieve high-efficiency degradation, and increase the effect of ethanol yield
CN109652325BActive Publication Date: 2022-05-13TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2022-05-13

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Abstract

The invention discloses an industrial strain of Saccharomyces cerevisiae (Saccharomyces cerevisiae) with delta integration, secretion and expression of cellulase and its application. A Saccharomyces cerevisiae industrial strain RδBEC with delta integration, secretion and expression of cellulase has a preservation number of CGMCC NO.16825. Application of the aforementioned industrial strain RδBEC, deposit number CGMCC NO.16825, in ethanol production. The bacterial strain RδBEC of the present invention, with the preservation number CGMCC NO.16825, secretes and expresses five kinds of cellulase: Trichoderma reesei CBH1, CBH2 and EG2; Aspergillus aculeatus BGL1 and CBH1. The bacteria can cooperate with existing commercialized cellulase to efficiently degrade cellulose and increase the yield of ethanol.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to an industrial strain of Saccharomyces cerevisiae that secretes and expresses various cellulases and its application. Background technique

[0002] The development and utilization of renewable energy is an active measure taken by human beings to face the problems of energy, environment and food. As the main bio-energy, fuel ethanol has attracted extensive attention due to its unique properties for vehicles. From 2005 to 2016, the global consumption of biofuel ethanol increased from 36.28 million tons to 79.15 million tons, with an average annual growth rate of 7.3%. At present, more than 40 countries and regions promote biofuel ethanol and ethanol gasoline for vehicles. The annual consumption of ethanol gasoline is about 600 million tons, accounting for about 60% of the total gasoline consumption in the world. In my country, in order to meet the growing energy ...

Claims

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