Unlock instant, AI-driven research and patent intelligence for your innovation.

A miRNA for distinguishing left and right colon cancer and its application

A technology for colon cancer and colon adenocarcinoma, applied in the field of molecular biology, can solve the problem of less research on the relationship between left and right colon cancer

Active Publication Date: 2022-08-02
天津市中西医结合医院
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only patents that report that miRNA is closely related to colon cancer, such as ZL2014101918129, CN2016106181508, and CN201610620059X, but there are few studies on the relationship between miRNA and colon cancer.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A miRNA for distinguishing left and right colon cancer and its application
  • A miRNA for distinguishing left and right colon cancer and its application
  • A miRNA for distinguishing left and right colon cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Screening of miRNAs related to distinguishing left and right colon adenocarcinoma

[0042] 1. Data collection

[0043] At the time of retrieval, the TCGA database included 460 patients with colon adenocarcinoma, including clinical data of 459 patients, mRNA data of 456 patients, and miRNA data of 444 patients.

[0044] Patients were screened for inclusion in the study according to clinical records. Require:

[0045] (1) histological_Type is colon adenocarcinoma;

[0046] (2) anatomic_neoplasm_subdivision is Ascending Colon, Sigmoid Colon, Cecum, Descending Colon.

[0047] A total of 314 patients were screened, including right colon adenocarcinoma including Ascending Colon and Cecum; left colon adenocarcinoma including Descending Colon and Sigmoid Colon.

[0048] The mRNA and miRNA data of colon adenocarcinoma transcriptome generated by TCGA database were used. After screening according to the above criteria, the samples without clinical information were d...

Embodiment 2

[0053] Example 2 Q-PCR verification of differentially expressed and miR-5586-5p

[0054] 1. Blood samples from 42 patients with colon cancer were collected from the Department of Gastrointestinal Surgery, Tianjin Nankai Hospital, including 20 samples from right colon cancer and 22 samples from left colon cancer. The samples were all pathologically confirmed.

[0055] 2. RNA extraction

[0056] Use QIAGEN Blood RNA Extraction Kit to extract RNA from serum. For specific steps, please refer to the instruction manual.

[0057] 3. Reverse transcription:

[0058] 1) Mix 10pg-1μg of total RNA template with 2μl 10X buffer, 2μl dATP (10mM), 0.5μl polyA polymerase, 0.5μl RNase inhibitor and RNase free water , the final volume was 20 μl, and incubated at 37°C for 1 h.

[0059] 2) Add 1 μl of 0.5 μg / μl Oligo(dT) specific RT primer to the reaction tube, and incubate at 70° C. for 5 min.

[0060] 3) Immediately incubate on ice for at least 2 min to disrupt the secondary structure of RN...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a miRNA for distinguishing left and right colon cancer and its application, in particular to miR-5586-5p and its application in preparing a diagnostic preparation for distinguishing left colon cancer and right colon cancer. Existing researches mostly focus on the development of molecular markers for the diagnosis of colon cancer, and there are few studies on the differentiation of left-sided colon cancer and right-sided colon cancer from genetic markers. The differential expression of 5586‑5p in left and right colon cancer is very obvious and verified, which provides a potential molecular marker for guiding the accurate diagnosis of colon cancer in different disease sites in clinic.

Description

technical field [0001] The invention relates to the field of molecular biology, and more particularly to miR-5586-5p and its application in preparing a diagnostic preparation for distinguishing left colon cancer and right colon cancer. Background technique [0002] Colon cancer is one of the most common malignant tumors of the digestive tract, and it mostly occurs at the junction of the rectum and the sigmoid colon, with the highest incidence in the 40-50-year-old age group. Its incidence ranks third among all human cancers. Bounded by the splenic flexure, the colon is divided into the right colon and the left colon. The former includes the ileocecal, ascending colon, hepatic flexure, and transverse colon; the latter is composed of the splenic flexure, descending colon, and sigmoid colon. Due to the different origins of the left and right colon embryos, there are differences in the physiological function, morphology and metabolism of the two, resulting in different parts of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178C12Q2600/112
Inventor 于向阳王西墨敖奇王彤彤马军宏杨士民李琳邹常林
Owner 天津市中西医结合医院
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com