Identification method of roundworms and application thereof
A technology for nematodes and Haemonchus contortus, applying a method for identifying nematodes and application fields thereof, can solve the problems of not finding worm eggs, distinguishing them, distinguishing worm eggs, etc., and achieves low cost, simple operation, and ease of use. Detecting Difficult Effects
Active Publication Date: 2019-04-26
SICHUAN ANIMAL SCI ACAD
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Problems solved by technology
However, because most of the eggs have very little difference in appearance, it is difficult to distinguish the eggs only by taking pictures under a microscope or their size. Distinctive traits that distinguish it from other worm eggs
Method used
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Embodiment 1
[0133] This embodiment provides a method for identifying nematodes, comprising the steps of:
[0134] 1. Collecting nematode eggs: collecting eggs by sedimentation method includes the following steps. Collect fresh fecal samples from cattle and sheep rectally. After crushing, wash and filter with an 80-mesh filter, discard the feces, and then filter the filtrate with a filter for 2~ 3 times, stand still for 20-30 minutes, pour off the supernatant; settle with clean water for 20-30 minutes, repeat this way 2-3 times to get eggs;
[0135] 2. Cultivate and observe the number of larvae and air chambers contained in nematode eggs: place the eggs in normal saline in an environment of about 25°C for 4 days, and observe the number and number of eggs containing larvae in the eggs under a microscope at 400 times. Contains the number of air cell eggs;
[0136] 3. Observe the appearance of nematode eggs (length, width and aspect ratio).
[0137] 4. Fluorescently labeled peanut agglutini...
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Abstract
The invention provides an identification method of roundworms and application thereof, and relates to the field of roundworm identification. The identification method of the roundworms includes the following steps that roundworm eggs are collected, wherein the roundworm eggs include haemonchus contortus eggs, osteragia eggs, chabertia ovina eggs and oesophagostomum eggs; by observing the amount oflarva eggs in the cultured roundworm eggs, the air chambers and appearances of the roundworm eggs and whether or not the roundworm eggs can be combined with peanut agglutinin, the haemonchus contortus eggs, the osteragia eggs, the chabertia ovina eggs and the oesophagostomum egg can be distinguished. The identification method of the roundworms is easy to operate and low in cost, the haemonchus contortus eggs can be accurately and efficiently distinguished from three other roundworm eggs, and the technical problems can be alleviated that haemonchus contortus in the animal body is difficult todetect, the detection cost is high, the operation is complex, and it is not easy to distinguish the haemonchus contortus eggs from the other roundworm eggs.
Description
technical field [0001] The invention relates to the field of nematode identification, in particular to a nematode identification method and application thereof. Background technique [0002] Common nematodes in the digestive tract of cattle and sheep include Haemonchus contortus, Osteria, Chabertus and esophageal stoma, among which Haemonchus contortus is the most pathogenic and most harmful to cattle and sheep. A parasitic nematode, this nematode mainly lives in the abomasum of cattle and sheep, occasionally in the small intestine, and feeds on the blood of the host. Currently popular methods for detecting parasitic diseases include egg detection methods, immunological detection methods and nucleic acid detection methods. These detection methods are very important for the differential diagnosis of Haemonchus contortus disease in cattle and sheep. Among them, the detection of eggs is intuitive, accurate, easy to operate, and can reflect the infection status of the host. It...
Claims
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Login to View More IPC IPC(8): G01N21/64G01N1/30A01K67/033
CPCA01K67/033G01N1/30G01N21/6458
Inventor 叶勇刚廖党金康润敏魏勇林毅谢晶叶建强戴卓建于吉峰曹冶李兴玉肖璐潘梦
Owner SICHUAN ANIMAL SCI ACAD