Method for Coating Antigen/Antibody on Microwell Plate Using Flat-bed Inkjet Printer

Abstract

A method of using a flat-panel inkjet printer to coat antigens / antibodies on a microporous plate, placing a clean microporous plate on the workbench of a flat-panel inkjet printer, adding reagents to a clean printer cartridge; obtaining pictures of the microporous plate, and In the computer software, the positions of the pictures corresponding to the microwells where the same reagent is added are set to the same color, and the reagents corresponding to the colors of the pictures are the same as the corresponding reagents in the corresponding ink cartridges; multiple reagents that need to be added in sequence are made into multiple files , print the pictures one by one in the corresponding order. In the present invention, the antigen / antibody is coated on the microporous plate by means of printer inkjet, so that the antigen / antibody can be stably and uniformly solidified, the operation is semi-automatic, and the dosage is accurate and uniform. By changing the picture information to change the coated quantity of the microplate and the concentration of antibody / antigen, it is convenient to set the concentration gradient of coated antigen / antibody to form a control group in ELISA, which is convenient for qualitative analysis and quantitative detection.

Description

technical field [0001] The invention relates to a method for coating antigen / antibody on a microporous plate, in particular to a method for coating antigen / antibody on a microporous plate by using a flat inkjet printer, and belongs to the field of biotechnology. Background technique [0002] Enzyme linked immunosorbent assay (ELISA) is the most widely used technology in enzyme immunoassay technology, based on the immobilization of antigen or antibody and enzyme labeling of antigen or antibody. It has been widely used in qualitative analysis and quantitative detection of various antigens and antibodies. [0003] In order to facilitate detection, many types of ELISA detection kits have been derived. Commercial ELISA kits are usually pre-coated in the microwells of a 96-well plate with a coating solution (antigen or antibody), and then the pre-coated microwell plate is blocked with a blocking solution to make a completely coated microwell plate. 96-well reaction plate for ant...

AI Technical Summary

Problems solved by technology

[0004] There are some problems in the current ELISA operation: 1. The microwell plate in the commercialized ELISA kit is coated with the same antibody / antigen, and only one item can be detected. If multiple items are to be detected, multiple different detection reagents or kits, and perform detection operations separately; 2. When adding reagents to the microwell plate, if the method of manual addition is used, the cost can be greatly reduced, but there are also inaccuracies in manual sample addition, and the operation is easy to fail. Errors and other factors, although the automatic enzyme immunoassay analyzer is easy to use, fully automatic, low maintenance, and pollution-free, it is expensive and the cost is too high; 3. When washing the microplate, if a general-purpose plate washer is used to wash the plate, it needs The whole plate is operated at the same time, while the manual plate washing needs to be filled with washing liquid one by one for washing, and the operation is extremely cumbersome

[0006] Although the above-mentioned patent uses automation to realize no waste of reagents, it needs to redesign a kind of machinery and equipment, and the cost is too high

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