Method for Coating Antigen/Antibody on Microwell Plate Using Flat-bed Inkjet Printer

An inkjet printer and antigen-coated technology, which is used in replication/marking methods, printing, instruments, etc., can solve the problems of inaccurate manual sample addition, error-prone operation, high price, etc., and achieve convenient qualitative analysis and quantitative detection. , high accuracy and precision, the effect of reducing pollution

Active Publication Date: 2022-02-18
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are some problems in the current ELISA operation: 1. The microwell plate in the commercialized ELISA kit is coated with the same antibody / antigen, and only one item can be detected. If multiple items are to be detected, multiple different detection reagents or kits, and perform detection operations separately; 2. When adding reagents to the microwell plate, if the method of manual addition is used, the cost can be greatly reduced, but there are also inaccuracies in manual sample addition, and the operation is easy to fail. Errors and other factors, although the automatic enzyme immunoassay analyzer is easy to use, fully automatic, low maintenance, and pollution-free, it is expensive and the cost is too high; 3. When washing the microplate, if a general-purpose plate washer is used to wash the plate, it needs The whole plate is operated at the same time, while the manual plate washing needs to be filled with washing liquid one by one for washing, and the operation is extremely cumbersome
[0006] Although the above-mentioned patent uses automation to realize no waste of reagents, it needs to redesign a kind of machinery and equipment, and the cost is too high

Method used

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  • Method for Coating Antigen/Antibody on Microwell Plate Using Flat-bed Inkjet Printer
  • Method for Coating Antigen/Antibody on Microwell Plate Using Flat-bed Inkjet Printer
  • Method for Coating Antigen/Antibody on Microwell Plate Using Flat-bed Inkjet Printer

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Experimental program
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Effect test

Embodiment 1

[0031] This embodiment is realized after refitting the Epson L805 inkjet printer. The base of the original printer is disassembled, a mobile working platform is added, and the paper pick-up motor of the printer is used to drive the movement of the working platform to convert it into a flatbed printer. The printer holds six color ink cartridges, light magenta, light cyan, yellow, magenta, cyan, black, so multiple reagents can be added at the same time.

[0032] In this example, a flat-panel inkjet printer was used to coat the anti-AFP antibody on a 96-well plate. In order to save reagents and avoid inaccurate operation results caused by spraying reagents onto the walls of the wells because the holes of the standard microplate plate were too deep, this paper The embodiment is completed with a commercially purchased 96-well plate cover, which greatly reduces the amount of reagents added (100 μL / well is required for traditional ELISA coating, and only 3-10 μL is required for inkjet...

Embodiment 2

[0040] In this embodiment, add the washing liquid to the clean ink cartridge corresponding to "yellow" and then put the ink cartridge into the correct position of the printer, set the corresponding position of the micropores that need to be washed to "yellow" in the image and save it as file 3. To implement Li No. 1 Middle School 4 o After overnight C, place the microwell plate back on the printer workbench and print file 3. It can be printed several times in a row so that the amount of washing liquid added exceeds the amount of coating liquid, so as to thoroughly clean the micropores. In subsequent washing operations is also like this. After printing, shake the microplate slightly, pour off the washing solution, pat it dry, and then place the microplate on the printer workbench. In Example 1, the microwell plate after adding the blocking solution at room temperature for two hours was washed in the same way to obtain a fully coated 96-well plate.

Embodiment 3

[0042] When two or more antibodies / antigens need to be coated, if anti-AFP antibody is coated in a part of wells of the same microwell plate, and AFP antigen is coated in another part of wells, anti-AFP antibody can be added Add the AFP antigen to the clean ink cartridge corresponding to "magenta", and place the ink cartridge in the correct position of the printer, and set the corresponding position of the microwell that needs to add anti-AFP antibody in the image to " Magenta", set the microwells that need to add AFP antigen to "black" in the corresponding position in the image and save it as file 4, print file 4 to add anti-AFP antibody and AFP antigen in different microwells at the same time. It is also possible to set the colors corresponding to the image corresponding to the anti-AFP antibody and the image corresponding to the AFP antigen respectively, save and print separately, that is, add different reagents to different microwells successively. When there are more than...

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Abstract

A method of using a flat-panel inkjet printer to coat antigens / antibodies on a microporous plate, placing a clean microporous plate on the workbench of a flat-panel inkjet printer, adding reagents to a clean printer cartridge; obtaining pictures of the microporous plate, and In the computer software, the positions of the pictures corresponding to the microwells where the same reagent is added are set to the same color, and the reagents corresponding to the colors of the pictures are the same as the corresponding reagents in the corresponding ink cartridges; multiple reagents that need to be added in sequence are made into multiple files , print the pictures one by one in the corresponding order. In the present invention, the antigen / antibody is coated on the microporous plate by means of printer inkjet, so that the antigen / antibody can be stably and uniformly solidified, the operation is semi-automatic, and the dosage is accurate and uniform. By changing the picture information to change the coated quantity of the microplate and the concentration of antibody / antigen, it is convenient to set the concentration gradient of coated antigen / antibody to form a control group in ELISA, which is convenient for qualitative analysis and quantitative detection.

Description

technical field [0001] The invention relates to a method for coating antigen / antibody on a microporous plate, in particular to a method for coating antigen / antibody on a microporous plate by using a flat inkjet printer, and belongs to the field of biotechnology. Background technique [0002] Enzyme linked immunosorbent assay (ELISA) is the most widely used technology in enzyme immunoassay technology, based on the immobilization of antigen or antibody and enzyme labeling of antigen or antibody. It has been widely used in qualitative analysis and quantitative detection of various antigens and antibodies. [0003] In order to facilitate detection, many types of ELISA detection kits have been derived. Commercial ELISA kits are usually pre-coated in the microwells of a 96-well plate with a coating solution (antigen or antibody), and then the pre-coated microwell plate is blocked with a blocking solution to make a completely coated microwell plate. 96-well reaction plate for ant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543B41M5/00
CPCG01N33/543B41M5/0047
Inventor 冯波许锦蒋子欣
Owner XIANGTAN UNIV
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