Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Barley hvhox9 gene and its use

A barley and gene technology, applied in the field of genetic engineering, can solve problems such as sensitivity to aluminum stress and restrictions on barley planting

Active Publication Date: 2020-06-19
ZHEJIANG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, barley is extremely sensitive to aluminum stress, which severely limits the cultivation of barley in many acidic soil agricultural regions around the world. Therefore, it is urgent to screen aluminum-tolerant germplasm and excavate aluminum-tolerant related genes to breed aluminum-tolerant barley varieties

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Barley hvhox9 gene and its use
  • Barley hvhox9 gene and its use
  • Barley hvhox9 gene and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Cloning and Analysis of CDS Region of HvHOX9 Gene

[0032] 1. Cloning of the CDS region sequence of HvHOX9 gene

[0033] Based on the previous research of the research group, a HD-ZIP family gene highly expressed in XZ16 was selected from the results of root miRNA and RNA-seq under aluminum stress, and the full-length CDS region sequence of the gene was cloned from XZ16 (such as SEQID NO.1), and named it HvHOX9.

[0034] Total RNA was extracted from the roots of the annual wild barley XZ16 on the Qinghai-Tibet Plateau using a total RNA extraction kit (Takara, Japan), and genomic DNA contamination in the total RNA was removed with DNaseI (Takara, Japan), and PrimeScript was used TMII 1stStrand cDNA Synthesis Kit Reverse Transcription Kit (Takara, Japan) reverse-transcribed the extracted total RNA into single-stranded cDNA. Design specific primers according to the sequence obtained by Blast, and the specific primer sequence is:

[0035] HvHOX9-CDS-F: 5'-ATGGCGGCGGCGGTG...

Embodiment 2

[0063] BSMV-VIGS method to verify the function of HvHOX9 gene

[0064] 1. Construction of BSMV:HvHOX9 vector

[0065] Using the pMD18-T-HvHOX9 plasmid as a template, primers were designed, and a 281bp HvHOX9 gene fragment was amplified.

[0066] KOD enzyme PCR reaction system:

[0067]

[0068] The amplification program was: 94°C for 5 min, (98°C for 10 s, 58°C for 30 s, 68°C for 30 s) 35 cycles, 68°C for 10 min.

[0069] The primer sequence is (the underline is the restriction site):

[0070] HvHOX9-γ-F: 5'-GTAC GCTAGC CTGCTGTTCTGCGGACATTT-3' (SEQ ID NO. 12);

[0071] HvHOX9-γ-R:5'-GTAC GCTAGC ATAATCAGCCCATTCAGACC-3' (SEQ ID NO. 13).

[0072] The HvHOX9 gene fragment was digested with NheI restriction endonuclease, and then ligated with the RNAγ vector that had been digested with the same restriction enzyme and dephosphorylated with T4 ligase. The ligation product was transformed into Escherichia coli DH5α, and the reverse insertion was verified with the forward p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a barley HvHOX9 gene and an application thereof in regulating barley aluminum resistance, and belongs to the technical field of gene engineering. The barley HvHOX9 gene has thenucleotide sequence of a CDS region shown in SEQ ID NO.1. Through cloning and analysis of the barley HvHOX9 gene and with combination of the BSMV-VIGS technology, through functional verification of the gene on XZ16, the HvHOX9 is found out to participate in regulating root system regeneration of XZ16 under aluminum tolerance, and reduce the aluminum content of cell walls. The theoretical basis and related genes are provided for barley aluminum tolerance breeding and production.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the barley HvHOX9 gene and its use in regulating the aluminum tolerance of barley. Background technique [0002] Aluminum is the most abundant metal element in the earth's crust, and aluminum mainly exists as insoluble compounds in soil. However, with the intensification of human activities, acid rain deposition and extensive use of fertilizers have led to serious soil acidification. According to statistics, acid soil accounts for about 50% of the world's arable land (Kochian et al., 2004). When the soil pH is lower than 5.0, aluminum will dissociate from the mineral state to the ionic state (in which Al 3+ The most toxic ion) is released into the soil, and the lower the pH, the greater the solubility of aluminum. Active aluminum has a toxic effect on plants, inhibiting root growth and water and nutrient absorption. Aluminum toxicity is one of the main factors limi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8271
Inventor 冯雪刘文星邬飞波张国平曹方彬王一州
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com