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A kind of fluorescent probe for pH detection, its synthesis method and application

A fluorescent probe and imaging technology, which is applied in the field of fluorescent probes for pH detection, can solve the problems of lack of effective means for pH, and achieve good selectivity, high sensitivity, and easy purification

Inactive Publication Date: 2021-06-15
INST OF PHARMACY SHANDONG PROV ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are fewer studies on strongly acidic (pH8.00)
So far, there is still a lack of effective means for pH detection in biological systems under such extreme acidic and alkaline conditions.

Method used

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  • A kind of fluorescent probe for pH detection, its synthesis method and application
  • A kind of fluorescent probe for pH detection, its synthesis method and application
  • A kind of fluorescent probe for pH detection, its synthesis method and application

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0037] Experimental example 1 (ultraviolet spectrum):

[0038] Acid-base titrations were performed systematically in PBS buffer-DMSO solution. The NDI sample was dissolved in PBS buffer: in a solution with a volume ratio of 4:1 of DMSO, and measured after standing for 30 min, as figure 1 As shown, there is a maximum absorption peak at 415nm and a side peak at 345nm under acidic conditions (pH 6). When the pH value gradually changed from 6.00 to 11.00, the maximum absorption peak was from 415 nanometers to 401 nanometers, and the absorption spectrum shifted blue with the increase of pH value, which was caused by ICT.

experiment example 2

[0039] Experimental example 2 (fluorescence spectrum):

[0040] Fluorescent pH titration of NDI probes such as figure 2 , 3 , 4 shown. The probe NDI shows a main emission band at 561 nm. When the pH value changed from 3.00 to 6.50, the fluorescence intensity at 561nm increased linearly. But when the solution changed from acidic to alkaline, that is, from 6.50 to 8.30, the fluorescence intensity increased rapidly, and there was no linear relationship between the change of fluorescence intensity and the pH value. The emission band at 561 nm of the probe increases linearly again as the pH increases from 8.30 to 10.50. Under strong acid conditions, the lone pair electron interaction between the nitrogen atom and the morpholine group disappears, electron transfer is inhibited, and the fluorescence intensity of the fluorescent mother nucleus decreases. The electron-donating ability of the morpholine group was restored with increasing basicity. At the same time, the hydrogen o...

experiment example 3

[0041] Experimental example 3 (selective experiment):

[0042] as attached Figure 5 As shown, in the presence of different ions, ROS or RNS in the solution of pH 7.2, the fluorescence when the probe (5mM) is excited at 561nm, it can be seen that the fluorescence intensity does not change.

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Abstract

The invention relates to a fluorescent probe for pH detection represented by the novel structural formula (I), its synthesis method and application, belonging to the fields of chemical synthesis and fluorescence detection. The fluorescent probe has a response site under acid-base conditions, can detect pH with high selectivity and sensitivity under both acid and alkaline conditions, and has important application value.

Description

technical field [0001] The invention relates to the fields of chemical synthesis and fluorescence detection, in particular to a fluorescent probe for pH detection, its synthesis method and its application. Background technique [0002] Intracellular pH (pHi) plays an important role in cellular events such as ion transport, muscle contraction, multidrug resistance, calcium regulation, cell growth, and apoptosis. Abnormal pHi can lead to many common diseases, including neurodegenerative diseases, Cystic fibrosis, Alzheimer's disease and cancer. In different prokaryotic species and in different subcellular compartments of eukaryotic cells, pHi values ​​can vary from highly acidic to basal. However, under the action of abnormal acid-base Candida, the channel proteins, transport proteins and signaling pathway proteins of the cell membrane will lose their normal functions. In addition, an abnormal acid-base environment will neutralize or degrade the functional groups of basic (a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D401/12C09K11/06G01N21/64
Inventor 王延风矫春鹏刘媛媛路文娟张平平李静
Owner INST OF PHARMACY SHANDONG PROV ACAD OF MEDICAL SCI
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