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T cell immunity enhancing composition and preparation method thereof

A technology of cellular immunity and composition, which is applied in the field of biomedicine and can solve the problems of limited methods for malignant tumors

Active Publication Date: 2019-05-24
REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current treatments for malignant tumors are limited

Method used

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  • T cell immunity enhancing composition and preparation method thereof
  • T cell immunity enhancing composition and preparation method thereof
  • T cell immunity enhancing composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 Bacteroides fragilis culture

[0049] training method

[0050] Step 1: Take a freeze-dried and preserved strain of Bacteroides fragilis (purchased from the ATCC official website), add 200 μL TSB medium, reconstitute, streak the blood plate, and put it in the anaerobic tank gas control system after pumping In a biochemical incubator at 37°C, anaerobic culture for 48 hours (h);

[0051] Step 2: Pick a monoclonal colony and insert it into 10ml TSB medium, and culture it anaerobically at 37°C for 12 hours;

[0052] Step 3: Take a bottle of 500ml TSB medium, insert 1% (v / v) strains, and culture anaerobically at 37°C for 48 hours;

[0053] Step 4: Take the bacterial solution and centrifuge at 6000rpm for 10min. Wash twice with normal saline, and finally reconstitute the sludge with normal saline for use and count the viable bacteria.

Embodiment 2

[0054] Example 2 Experiment of the therapeutic effect of Bacteroides fragilis on tumors in mice (for example: melanoma)

[0055] figure 1 Schematic diagram of the experimental flow for detecting the therapeutic and / or preventive effects of Bacteroides fragilis and inactivated Bacteroides fragilis on mouse tumors (melanoma).

[0056] 1. Training method

[0057] The culture method of Bacteroides fragilis is the same as in Example 1.

[0058] 2. Sample preparation

[0059] 1) Preparation of live Bacteroides fragilis ZY-312 cells

[0060] Step 1: Take a freeze-dried strain (purchased from the official website of ATCC), add 200 μL of freeze-dried culture medium, redissolve, draw 20 μL, draw a line on the blood plate, and perform biochemical culture after the gas control system of the anaerobic tank is pumped In the box at 37°C, anaerobic culture for 48h;

[0061] Step 2: Pick a monoclonal colony and insert it into 10ml TSB medium, and culture it anaerobically at 37°C for 12 ho...

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Abstract

The invention provides a T cell immunity enhancing composition. The T cell immunity enhancing composition comprises bacteroides fragilis. The invention also discloses a tumor preventing and / or treating composition. The tumor preventing and / or treating composition comprises bacteroides fragilis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a composition for enhancing T cell immune function, a composition for preventing and / or treating tumors, and a preparation method of the composition. Background technique [0002] Malignant tumors have become the "No. 1 killer" that threatens human health and life. Among the causes of human death, the number of deaths from malignant tumors has risen to the top. About 8.7 million people in the world suffer from tumors every year, and about 6.9 million people die. In my country alone, 1.05 million people die of advanced malignant tumors every year. Current treatments for malignant tumors are limited. [0003] Bacteroides fragilis (B. fragilis for short) is a Gram-negative, rod-shaped, blunt-rounded ends and densely stained, capsulated, non-spored, non-motive obligate anaerobic bacteria, which can be divided into Enterotoxigenic and non-enterotoxigenic types. Bacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P37/04A61P35/00A23L33/135
CPCA23L33/135A61K35/74A61P35/00A61P37/00A61P37/04C12R2001/01A23V2002/00C12N1/20A23V2200/308A23V2250/206A23V2200/324C12N1/205
Inventor 曾谷城
Owner REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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