obr gene and its method for use as a genetic marker related to fat deposition in fat liver of Xupu goose

A genetic marker, Xupu goose technology, used in biochemical equipment and methods, determination/inspection of microorganisms, DNA/RNA fragments, etc.

Active Publication Date: 2022-06-21
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on DNA genetic markers, genetic diversity and related functional genes of Xupu goose is still in its infancy, and the genetic markers related to fatty liver fat deposition in Xupu goose have not been reported yet.

Method used

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  • obr gene and its method for use as a genetic marker related to fat deposition in fat liver of Xupu goose
  • obr gene and its method for use as a genetic marker related to fat deposition in fat liver of Xupu goose
  • obr gene and its method for use as a genetic marker related to fat deposition in fat liver of Xupu goose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 SNP screening of genes related to liver fat deposition in Xupu geese

[0048] 60 healthy Xupu geese of the same age were selected for feeding experiment. After 28 days for slaughter, Xupu goose livers were weighed and their genomic DNA was extracted from extreme samples (large and small livers).

[0049]According to the goose OBR gene sequence in GenBank, primers were designed with Primer 5.0, covering all exon 14 to exon 16 sequences of the gene. The primer sequences are: 5'-TTTGCCGTTAGTACCAGG-3' (forward), 5'-GCACAGACAAACAGGAGC- 3' (reverse), synthesized by Hunan Qingke Biotechnology Co., Ltd. The Xupu goose genomic DNA was amplified with the above primers.

[0050] PCR: 20 μl reaction system: 12 μl of 2×Master mix, 0.8 μl of upstream and downstream primers (10 μmol / L)), 1 μl of DNA template, and supplemented with ultrapure water to the required volume.

[0051] PCR amplification procedure: pre-denaturation at 95 °C for 5 min; denaturation at 95 °C for 30...

Embodiment 2

[0055] Example 2 Verification of SNPs related to liver fat deposition in Xupu goose in production practice

[0056] 14 healthy Xupu geese at 80 days of age and under the same feeding and management conditions were selected for the experiment. After 28 days of feeding, all the tested Xupu geese were slaughtered, and their livers were weighed.

[0057] 14 Xupu geese liver tissue-like genomic DNAs were extracted and amplified using the primers in Example 1, and their SNPs were detected according to the method in Example 1.

[0058] 1. PCR

[0059] The PCR reaction system is shown in the following table:

[0060] Table 1 PCR reaction system

[0061]

[0062] PCR cycling parameters are as follows:

[0063] Pre-denaturation at 95 °C for 5 min; denaturation at 95 °C for 30 s, annealing at 57 °C for 30 s, extension at 72 °C for 90 s, a total of 35 cycles; extension at 72 °C for 10 min, and final storage at 4 °C.

[0064] 2. Genotype frequency analysis of OBR gene SNP loci

[...

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Abstract

The invention discloses an OBR gene and a method for acting on Xupu goose liver fat deposition-related genetic markers, using the leptin receptor gene OBR and its genetic markers as the traits of Xupu goose fat liver, by measuring the size of Xupu goose fat liver , and screen the OBR gene polymorphisms of Xupu geese, and then analyze the association between the OBR gene polymorphisms and the traits of fatty liver. The sequence of the OBR molecular marker is shown in SEQ ID NO:1. There is a G / A base mutation at 573bp of SEQ ID NO:1, and the liver size of Xupu goose with GG genotype is significantly higher than that of individuals with GA genotype. The invention provides molecular markers for marker-assisted selection of Xupu goose fat liver traits.

Description

technical field [0001] The invention belongs to the field of poultry molecular biotechnology and breeding, and mainly relates to the determination of genetic markers related to the fatty liver traits of Xupu goose, specifically, to determine the polymorphism in the OBR gene, that is, the determination of the polymorphisms related to the fatty liver traits of Xupu goose The presence of a single nucleotide polymorphism (Singlenucleotide polymorphism, abbreviated as SNP) in the leptin receptor gene. [0002] technical background [0003] Xupu goose is a national-level local breed genetic resource with the best fat liver performance, large size, fast growth, strong disease resistance and obvious advantages in my country. In 2014, it entered the national livestock and poultry genetic resources protection list. Goose fat liver is one of the three treasures in the world-class high-end delicious healthy food. Its rich unsaturated fatty acids, linoleic acid, lecithin, etc. have import...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 曲湘勇柳序郭松长贺长青
Owner HUNAN AGRICULTURAL UNIV
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