Mathematical sequence reconstruction method for long-chain molecules

Abstract

The invention relates to a long-chain molecule sequence mathematical reconstruction method, and specifically relates to a mathematical sequence reconstruction method for long-chain molecules, for geneDNA sequencing, protein amino acid sequencing or other long-chain structural chemical detection. The mathematical sequence reconstruction method for long-chain molecules includes the steps: 1) providing at least two DNA molecular chains, 2) breaking the at least two DNA molecular chains into fragment sequences to form X gene libraries, 3) sequencing gene fragments of the gene libraries to obtainlibrary fragment information; 4) performing full arrangement and splicing on the fragments of the library fragments, and obtaining a set of possibilities; 5) solving an intersection; and 6) judging the number of elements in the intersection to get a correct gene sequence map. The mathematical sequence reconstruction method for long-chain molecules belongs to a mathematical algorithm technology, and can realize sequencing and reconstruction of gene sequences, and sequencing and reconstruction of proteins, polysaccharides or other polymers having a single structure, thereby improving the accuracy in current gene sequencing, and the mathematical sequence reconstruction method for long-chain molecules is not probability speculation, but is based on rigorous mathematical algorithms, thus beinghigh in accuracy of sequencing structure.

Description

technical field

[0001] The invention relates to a long-chain molecular sequence mathematical reconstruction algorithm, in particular to a mathematical sequence reconstruction method for long-chain molecules used for gene DNA sequence determination, protein amino acid sequence determination or other long-chain structural chemical substance detection. Background technique

[0002] Sequence detection is involved in both biology and materials science, that is, the determination of various group arrangements of a certain chain, such as protein sequence determination, DNA sequence determination, polysaccharide sequence determination, etc. For example, to illustrate the bottlenecks faced in sequence determination, in molecular biology research, DNA sequence analysis is the basis for further research and transformation of target genes.

[0003] The current technologies used for sequencing mainly include the dideoxy chain terminal termination method invented by Sanger et al. (1977) a...

Images