A strain of Bacillus atrophaeus effective against Lycium barbarum leaf blight, biological agent and its application

A technology of Bacillus atrophaeus and biological agent, applied in the direction of biocide, application, biocide, etc., to achieve the effect of good control, inhibition of germination, and wide application range

Active Publication Date: 2020-09-25
武威市林业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been many reports on the application of antagonistic microorganisms to control plant diseases, but there are few reports on antagonistic microorganisms that can effectively control Lycium barbarum leaf blight at home and abroad.

Method used

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  • A strain of Bacillus atrophaeus effective against Lycium barbarum leaf blight, biological agent and its application
  • A strain of Bacillus atrophaeus effective against Lycium barbarum leaf blight, biological agent and its application
  • A strain of Bacillus atrophaeus effective against Lycium barbarum leaf blight, biological agent and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The isolation and screening method and identification of the antagonistic Bacillus LKYLW-5 in this example are as follows. The strains in this example were collected from the cultivated soil in the Lycium barbarum planting area of ​​Changcheng Township, Liangzhou District, Wuwei City.

[0045] 1. Screening of antagonistic bacillus LKYLW-5, the screening process comprises the following steps:

[0046] 1) Weigh 10g of the cultivated soil in the wolfberry planting area collected, add it to a 250mL Erlenmeyer flask filled with 90mL of sterile water and glass beads, bathe in 80°C water for 30min, shake fully for 30min, dilute by 10 times, and use Spread a sterile spreader on the LB medium plate, and use sterile water as a control. Gram staining and spore staining of the purified bacteria showed that the bacteria were rod-shaped, spore-forming, and G+ isolates were Bacillus. Number the bacillus isolated by the above method, and observe the colony morphology. Each 1000mL LB ...

Embodiment 2

[0063] The antagonism of the antagonizing Bacillus LKYLW-5 strain of this example on Lycium barbarum leaf blight was tested as follows.

[0064] Confrontation culture test: put fresh Lycium barbarum blight bacteria with a diameter of 5 mm (obtained by the applicant from isolated and identified diseased plants in the Lycium barbarum planting area of ​​Changcheng Township, Liangzhou District, Wuwei City) in the center of the PDA plate, and after culturing at 28°C for 24 hours, The antagonistic Bacillus LKYLW-5 strain to be tested was connected around the equidistant distance from the fungus, and the control group was not inoculated with the antagonistic Bacillus LKYLW-5 strain; the PDA plate was placed in a 28°C incubator for confrontation culture, and after 5 days of cultivation, Observe the antagonistic effect of the antagonistic Bacillus LKYLW-5 strain.

[0065] The results of the confrontation culture test found that the growth of the control group leaf blight bacteria was n...

Embodiment 3

[0067] Effects of antagonistic Bacillus LKYLW-5 strain on the hyphae of the pathogen Alternaria of Lycium barbarum leaf blight.

[0068] Using the plate confrontation method, the pathogenic fungus (the applicant obtained from the separation and identification of the diseased plants in the Lycium barbarum planting area of ​​Changcheng Township, Liangzhou District, Wuwei City) bacteria cake was inoculated in the center of the PDA plate, and the two sides 3 cm away from the center were inoculated and cultivated for 48 hours and grew well. Antagonistic Bacillus LKYLW-5 strain was cultured upside down at 28°C for 4 days. Observe the mycelium growth at the edge of the inhibition zone near the bacillus, and use the edge mycelium far away from the antagonistic bacillus as a control to observe the growth of the mycelium microscopically, with more than 3 fields of view each time. The results show that: the growth of the control mycelium is fine Long and uniform, the cell structure is re...

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Abstract

The invention discloses bacillus with a boxthorn leaf blight preventing effect. The bacillus is antagonistic bacillus LKYLW-5 and belongs to bacillus atrophaeus, the length of a 16 S rDNA nucleotide sequence of the bacillus is 1492bp, the bacillus is preserved in China General Microbiological Culture Collection Center on November 29th 2018, and a preservation number is CGMCC NO.16837. The bacillusdisclosed by the invention is obtained from cultivated soil of a boxthorn planting area through slab confrontation culture screening; the bacillus can be applied to preventing and treating boxthorn leaf blight caused by alternaria alternata; the bacillus can inhibit mycelia growth of boxthorn leaf blight bacteria and germination of alternaria alternata spores; after boxthorn leaf blight pathogenic bacteria are inoculated to boxthorn plants, a preventing effect is 62.96%, and a treating effect is 60.15%; the bacillus has high-efficiency and obvious preventing and treating effects on the boxthorn leaf blight, is a biological prevention and control potential strain with high preventing efficiency and good environmental safety and has good development and application prospects.

Description

technical field [0001] The invention relates to the field of bacterial strains, in particular to a bacillus atrophaeus effective for preventing Lycium barbarum leaf blight, a biological bacterial agent and an application thereof. Background technique [0002] Lycium barbarum is not only a traditional precious Chinese herbal medicine, but also a nutritional tonic. In the list of 63 kinds of medicine and food dual-use announced by the Ministry of Health, it ranks first. As a traditional Chinese medicine, wolfberry has a flat taste and a sweet taste, which has the effects of nourishing the liver and kidney, improving eyesight and nourishing the lungs. Through chemical composition analysis, the fruit of wolfberry is detected to be rich in natural carotene, vitamin C, wolfberry proteoglycan, betaine, linoleic acid, iron, phosphorus, calcium and other nutrients. Nourishes the kidneys and nourishes the lungs, protects the liver and fights tumors. [0003] Lycium barbarum has bee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/22A01P3/00C12R1/07
Inventor 刘伟张军何彩王多文曹虎史星雲金娜李强张勤德王鑫赵连鑫郭艳兰张强威李栋董存元姚元文
Owner 武威市林业科学研究院
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