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An electrochemical aptamer sensor for acetamiprid based on hollow porous nanospheres with dual amplification signals

An aptamer sensor and a signal-amplifying technology, which can be used in electrochemical variables of materials, scientific instruments, instruments, etc., to solve the problems of inability to detect acetamiprid, difficulty in on-site emergency monitoring and online analysis, and many supporting equipment.

Inactive Publication Date: 2021-07-06
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a large amount of organic solvent is required, which is easy to cause environmental pollution
[0012] The above methods generally require pre-treatment of samples, which is cumbersome and time-consuming, requires a lot of supporting equipment, makes it difficult to realize on-site emergency monitoring and online analysis, and easily causes environmental pollution
Moreover, due to various reasons, the selectivity and detection limit of many methods are not ideal, and it is impossible to detect trace or even trace amounts of acetamiprid

Method used

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  • An electrochemical aptamer sensor for acetamiprid based on hollow porous nanospheres with dual amplification signals
  • An electrochemical aptamer sensor for acetamiprid based on hollow porous nanospheres with dual amplification signals

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Experimental program
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Embodiment 1

[0038] 1. Preparation of Acetamiprid Electrochemical Aptamer Sensor Signal Label

[0039] (1) Preparation of polyethyleneimine-ferrocene complex (PEI-Fc): Weigh 0.36g ferrocene formate and mix with 25mL ultrapure water, add 2.5mL cross-linking agent EDC / NHS solution, stirred for 2 hours; then added 60 μL of amino-terminated polyethyleneimine (PEI) overnight, centrifuged at 8000 rpm for 15 minutes, and then washed twice with ultrapure water; finally dispersed with 6 g of water, stored in the refrigerator, and prepared A concentration of 1% PEI-Fc was obtained;

[0040] (2) Solid SiO 2 Preparation of nanospheres: Mix 10mL of ammonia water, 16.5mL of absolute ethanol and 25mL of ultrapure water in a three-neck flask, as liquid A; mix 4.5mL of tetraethyl silicate with 45mL of absolute ethanol, as liquid B ; Mix liquid B and liquid A in a clean three-necked flask, let it stand for 30s, seal it with a parafilm, adjust the speed of the magnetic stirrer to 400rpm, and stir the solu...

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Abstract

The invention relates to the technical field of electrochemical sensors, in particular to a method for preparing an electrochemical aptamer sensor for detecting pesticide residue acetamiprid based on hollow porous silica polymer nanospheres, including acetamiprid electrochemical adaptation The preparation steps of the body sensor, and the operation method of using the sensor to measure acetamiprid, etc., this acetamiprid electrochemical aptamer sensor with dual signal amplification based on hollow porous silica polymer nanospheres, material synthesis The method is simple and convenient, the sensor is easy to make, the material is cheap, the sensor is easy to update, the reproducibility is good, the method is non-toxic, does not pollute the environment, and the determination method has good selectivity and high sensitivity.

Description

technical field [0001] The present invention relates to the technical field of electrochemical sensors, in particular to a method for preparing an electrochemical aptamer sensor based on a hollow porous silica composite to construct a double-amplified signal for the detection of pesticide residue acetamiprid and using the sensor to detect acetamiprid Specific methods for amidines. Background technique [0002] The problem of food contamination caused by pesticide residues has attracted widespread attention around the world. More and more deaths caused by pesticide poisoning and diseases caused by pesticide residues have sounded the alarm for people on food safety issues again and again. . Acetamiprid is a new type of insecticide, which belongs to organic insecticides. Its insecticidal mechanism is to paralyze the nervous system of pests, affect the stimulation transmission of the nervous system of pests, and lead to the blockage of nervous system pathways, so that neurotran...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/48
Inventor 刘汉彪周长利夏方诠陈培培刘建辉
Owner UNIV OF JINAN