DNA storage method based on oligonucleotide sequence code storage

Abstract

The invention relates to a DNA storage method based on oligonucleotide sequence code storage. The DNA storage method based on oligonucleotide sequence code storage performs oligonucleotide sequence coding on different binary string segments in a variety of different base coding formats, and each set of binary coded file fragments can always find an oligonucleotide sequence code that satisfies therequirements for synthesis and sequencing, thus greatly simplifying coding of binary string segments of oligonucleotides and the conversion of coded oligonucleotides into binary string segments for operation, realizing efficient coding and decoding operations, and maximally utilizing the data storage capacity of DNA molecules while simplifying codes of binary string segments and the operation of restoring the coded oligonucleotide sequence to binary string segments. In addition, in coded binary character segments, the maximum theoretical value of each base coded binary is 2 bytes, thus reducing the number or length of oligonucleotide sequences, reducing the cost of oligonucleotide synthesis and sequencing, and having the largest information storage amount.

Description

technical field [0001] The invention relates to a DNA storage method based on oligonucleotide sequence code storage, belonging to the technical field of information storage. Background technique [0002] Existing documents such as characters and pictures are stored on storage media such as hard disks and magnetic tapes after adopting binary coded documents in the digital storage process. Human beings generate 2.5quintillion (million cubic) bytes of data every day, and this speed is still accelerating. IBM estimates that 90% of the data in the world is generated in the past two years. As human life becomes more and more More digital storage is used, and this trend will only continue to expand. The current storage devices can no longer meet the demand for huge amounts of data, so people start to use new storage media. [0003] DNA is often referred to as the "blueprint of life" for obvious reasons. Every cell in the human body is encoded by a sequence of bases (A, G, C, and ...

AI Technical Summary

Problems solved by technology

However, existing algorithms (such as Nature Biotechnology, 2018, 36(3), 242-248; Science, 2017, 355, 950–954, etc.) are encoding oligonucleotide sequences to meet the requirements of synthesis and sequencing oligonucleotide sequences , when realizing the storage of DNA molecules, the existing encoding methods either reduce the data storage capacity of DNA molecules (the maximum theoretical information storage capacity of DNA molecules is 2 bytes of a base storage binary file: if the quaternary DNA storage 4m The information is the same as the traditional binary storage 2k information, then m=k / 2); or the calculation is complicated and time-consuming when the sequence is encoded and the encoded sequence is restored to the original sequence

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