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Sugar-free tissue culture method for increasing plant rooting rate

A technology for plant tissue culture and rooting rate, applied in the directions of botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of reducing the survival rate of bottled seedlings, non-standard tissue culture methods, and easy contamination of culture medium, and achieves improvement. The effect of bottle seedling growth environment, strengthening normal bottle seedling growth, and improving survival rate

Inactive Publication Date: 2019-06-11
陕西海棠生态农林股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Plant sugar-free tissue culture technology is based on CO 2 It replaces the sugar in the culture medium as a carbon source, adopts the method of artificially controlling the plant growth environment, and provides a suitable growth environment for the plant. It has the advantages of short cultivation period, unlimited regions and seasons, etc., and is widely used, but the plants are sugar-free There are still some problems in the implementation of tissue culture technology. For example, the rooting of bottle seedlings is affected due to non-standard tissue culture methods and easy contamination of the medium, thereby reducing the survival rate of bottle seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A plant sugar-free tissue culture method, comprising the following steps:

[0041] 1) Prepare bottle seedlings and clean and dry tissue culture bottles, culture boxes, trachea

[0042] Clean the tissue culture bottle, culture box and trachea with detergent, and dry them in a blast drying oven at 60°C for later use; use a culture box with 6 air holes;

[0043] 2) Material preparation

[0044] 2.1) Use vermiculite with a particle size of 3 mm to 8 mm as the matrix, add water to the vermiculite so that the mass percentage of water is 55%, mix well, put it into a canvas bag, and use a high-pressure steam sterilizer at 121 ° C for 20 minutes ;

[0045] 2.2) In the 1 / 2MS medium that does not contain organic matter, add the special antibacterial agent for plant tissue culture according to the standard of 0.67g / L, and add IAA according to the standard of 0.8mg / L; Keep the steam sterilizer at 121°C for 20 minutes to make a culture solution;

[0046] 3) subpack the substrate ...

Embodiment 2

[0058] A plant sugar-free tissue culture method, comprising the following steps:

[0059] 1) Prepare bottle seedlings and clean and dry tissue culture bottles, culture boxes, trachea

[0060] Clean the tissue culture bottle, culture box and trachea with detergent, and dry them in a blast drying oven at 60°C for later use; use a culture box with 6 air holes;

[0061] 2) Material preparation

[0062] 2.1) Use vermiculite with a particle size of 3 mm to 8 mm as the matrix, add water to the vermiculite so that the mass percentage of water is 45%, mix well, put it into a canvas bag, and use a high-pressure steam sterilizer at 124 ° C for 15 minutes ;

[0063] 2.2) In the 1 / 2MS medium that does not contain organic matter, add the special antibacterial agent for plant tissue culture according to the standard of 0.3g / L, and add IAA according to the standard of 1.0mg / L; The steam sterilizer is kept at 118°C for 25 minutes to make a culture solution;

[0064] 3) subpack the substrat...

Embodiment 3

[0076] A plant sugar-free tissue culture method, comprising the following steps:

[0077] 1) Prepare bottle seedlings and clean and dry tissue culture bottles, culture boxes, trachea

[0078] Clean the tissue culture bottle, culture box and trachea with detergent, and dry them in a blast drying oven at 60°C for later use; use a culture box with 6 air holes;

[0079] 2) Material preparation

[0080] 2.1) Use vermiculite with a particle size of 3mm to 8mm as the matrix, add water to the vermiculite so that the mass percentage of water is 35%, mix well, put it into a canvas bag, and keep it at 118°C for 25min in a high-pressure steam sterilizer ;

[0081] 2.2) In the 1 / 2MS medium that does not contain organic matter, add a special antibacterial agent for plant tissue culture according to the standard of 0.3g / L, and add IAA according to the standard of 0.5mg / L; The steam sterilizer was kept at 124°C for 15 minutes to make a culture solution;

[0082] 3) subpack the substrate i...

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Abstract

The invention provides a sugar-free tissue culture method for increasing the plant rooting rate. The rooting rate and survival rate of bottle seedlings can be increased. The method comprises the stepsof preparation of the bottle seedlings, tissue culture bottles, culture boxes, air pipes, mediums, and a culture solution, filling of the culture boxes, seedling planting, sugar-free room culture, and seedling hardening. According to the sugar-free tissue culture method, the operation is normal, no organic matter is added in the culture mediums, a bacteriostatic agent special for plant tissue culture is added, the growth environment of the bottle seedlings is greatly improved, rooting and growth of the bottle seedlings are facilitated, and the survival rate of the bottle seedlings is increased.

Description

technical field [0001] The invention belongs to sugar-free culture micropropagation, in particular to a sugar-free tissue culture method for improving plant rooting rate. Background technique [0002] Plant sugar-free tissue culture technology is based on CO 2 It replaces the sugar in the culture medium as a carbon source, adopts the method of artificially controlling the plant growth environment, and provides a suitable growth environment for the plant. It has the advantages of short cultivation period, unlimited regions and seasons, etc., and is widely used, but the plants are sugar-free There are still some problems in the implementation of tissue culture technology. For example, the rooting of bottle seedlings is affected due to non-standard tissue culture methods and easy contamination of medium, thereby reducing the survival rate of bottle seedlings. Contents of the invention [0003] The object of the invention is to provide a plant sugar-free tissue culture method...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王忠景郭瑶靳海燕郑燕青孙彪孙维维高天喜程娜
Owner 陕西海棠生态农林股份有限公司
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