Monoclonal antibody protection solution and its preparation method and application, reagents and immunohistochemical kits for applying monoclonal antibody protection solution
A monoclonal antibody and protective solution technology, applied in biological testing, instruments, measuring devices, etc., can solve problems such as high cost, poor antibody stability, and inability to accurately guide diagnosis
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[0072] The present invention also provides the preparation method of the above monoclonal antibody protective liquid, the preparation method comprising: each of the components of the acquired equivalent is aqueous solution to obtain the monoclonal antibody protector.
[0073] The method is simple, easy to operate, and the preparation of the obtained monoclonal antibody protective liquid is stable, and the antibody titer can be effectively maintained.
[0074] In some specific embodiments, the preparation method of the above monoclonal antibody protective liquid is as follows:
[0075] (a) 10mmol / L PBS
[0076] Mother liquor configuration: 371.6G NA 2 HPO 4 -12h 2 O, dissolve in 1000 ml of water, 5.2 m mother liquor;
[0077] Take 50ml of 0.2 M mother liquor, add water to 1000ml;
[0078] (b) 1 ‰ TWEEN20
[0079] Weigh 1 g tween20, soluble in 1000 ml 10 mmol / l PBS;
[0080] (c) 5% -10% goat serum (bovine serum)
[0081] Take 50-100ml of goat serum in 1000 ml 10 mmol / l PBS;
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Embodiment 1
[0122] This example provides a monoclonal antibody of N1, N12-acetyl amine amine, including:
[0123] 5mmol / l PBS, 2 ‰ (w / v) Tween 20, 1% (V / V) serum, 10% (w / v) trehalose, 0.1 ‰ (v / v) prolin, 2% (w / v) glycerin 0.1% (w / v) 6-amino acetic acid, 80 mmol / l EDTA and 0.1% (w / v) Casein-Na.
Embodiment 2
[0125] This example provides a monoclonal antibody of N1, N12-acetyl amine amine, including:
[0126] 15mmol / l PBS, 0.1 ‰ (w / v) Tween20, 15% (V / V) serum, 1% (w / v) trehalose, 2 ‰ (v / v) prolin, 0.5% (w / v) glycerin 1% (w / v) 6-amino acetic acid, 20 mmol / l EDTA and 0.5% (w / v) casein-Na.
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