Multi-dimensional liquid chromatogram separation system based on dual two-position four-way valve

Abstract

The invention provides a multi-dimensional liquid chromatogram separation system comprising a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradientpump B, a high performance liquid phase dilution liquid pump, a gradient mixer A, a gradient mixer B, a sampling valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a dual two-position four-way valve and a connecting pipeline. With switching of the dual two-position four-way valve, conversion betweena last one-dimensional separation state and a next one-dimensional separation state is realized and thus three-dimensional or over three-dimensional chromatographic separation is realized. The liquidchromatography separation column array is used for selecting all-dimensional chromatographic separation columns; and with the same gradient elution system and the same detector, all on-line monitoringand controlling of multi-dimensional chromatographic separation as well as the cleaning degree controllability of the enrichment column and the separation column are realized. All-dimensional separation is connected by the enrichment columns and compound enrichment or capturing is assisted by using the diluent pump. According to the invention, high-efficiency separation of the monomeric compoundin the complex system sample with the high separation difficulty is realized by selecting different chromatographic stationary phase and mobile phase combinations.

Description

technical field

[0001] The invention belongs to the technical field of high performance liquid chromatography separation, and relates to a multidimensional liquid chromatography separation system. Background technique

[0002] With the development of separation technology, exploring and separating components in complex sample systems has become a hot research field. Multidimensional liquid chromatography technology can effectively improve the resolution of complex sample component separation by increasing peak capacity, and has become the development direction of fast chromatography separation technology. Multidimensional liquid chromatography technology is a liquid chromatography technology that sequentially injects the eluent of the first-dimensional chromatographic column of the sample into the subsequent dimensional chromatographic column for further separation. This separation technology can utilize two or more chromatographic columns with different separation mechanis...

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