Application of protein OsARE1 in regulating plant senescence

A plant senescence, protein technology, applied in applications, plant peptides, plant genetic improvement, etc.

Active Publication Date: 2019-06-21
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another major challenge in the field of leaf senescence research is to assess the application prospects of genes controlling senescence

Method used

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  • Application of protein OsARE1 in regulating plant senescence
  • Application of protein OsARE1 in regulating plant senescence
  • Application of protein OsARE1 in regulating plant senescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0129] Embodiment 1, the genetic screening of abc1-1are1-1 double mutant

[0130] Cytokinins are an important class of plant hormones that participate in the regulation of various aspects of plant growth and development, including delaying leaf senescence. The inventors of the present invention screened a mutant with abnormal cytokinin response through chemical mutagenesis of rice variety Nipponbare (NPB), and named it abnormal cytokinin reponse1 mutant (abc1-1 mutant) (Yang et al. al., 2016). The abc1-1 mutant has obvious growth and development defect phenotypes, including plant dwarfing, reduced tiller number, and yellow leaves. lc7, early senescence7 (es7) and spotted leaf32 (spl32) (Agron. J. Zeng et al., 2017; Bi et al., 2017; Chen et al., 2016; Sun et al., 2017). The ABC1 gene encodes a chloroplast-localized Fd-GOGAT protein, which is a key enzyme that catalyzes nitrogen assimilation. The results of the study showed that the ABC1 / Fd-GOGAT gene function deficient mutan...

Embodiment 2

[0132] Embodiment 2, the phenotype analysis of are1-1 single mutant

[0133] In order to obtain the are1-1 single mutant, the inventors of the present invention backcrossed the abc1-1are1-1 double mutant with the rice variety Nipponbare three times to obtain BC 2 f 2 Separate groups. by BC 2 f2 Segregate populations for phenotype and linkage analysis to obtain the are1-1 single mutant.

[0134] Compared with the rice variety Nipponbare, the are1-1 single mutant had no obvious phenotypic differences in the vegetative growth stage ( figure 2 middle a, b and c); after entering the heading stage, the plant height of the are1-1 single mutant gradually increased, the number of tillers slightly decreased, the heading stage was delayed by about 3 to 5 days, and the chlorophyll content of the leaves increased significantly ( figure 2 middle d and e). In order to clarify whether the are1-1 mutation affects the leaf senescence process, the inventors of the present invention analyz...

Embodiment 3

[0135] Embodiment 3, map-based cloning of OsARE1 gene

[0136] In order to determine the nature of the are1-1 mutation, the inventors of the present invention performed a backcross analysis of the are1-1 single mutant with the rice variety Nipponbare. 18 hybrid F strains obtained by backcrossing 1 Progeny plants all showed the phenotype of rice variety Nipponbare, and in F 2 Plants with normal and late senescence phenotypes in segregating populations exhibited a phenotypic segregation ratio close to 3:1 (561:196 = 2.86:1; χ 2 c2 0.05 = 3.84). Thus, the are1-1 single mutant is caused by a recessive mutation of a single nuclear gene.

[0137] The inventors of the present invention isolated and identified the candidate OsARE1 gene by map-based cloning method. Genetic mapping populations were constructed using the abc1-1are1-1 double mutant and indica rice varieties Minghui 63 and Nanjing 6, respectively. Taking the late senescence phenotype of leaves as the selection index,...

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Abstract

The invention discloses application of protein OsARE1 in regulating plant senescence. The protein OsARE1 is a protein having an amino acid sequence which is shown as sequence 1 in a sequence table. Experiments prove that an encoding gene of the protein OsARE1 in rice is silenced or knocked off, so that plant senescence can be retarded. The protein OsARE1 and its encoding gene are an effective genetic target to improve the ability of plants to resist senescence and related traits, and have potential market value and a good application prospect in the field of agriculture. The application has important applicable value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of protein OsARE1 in regulating plant aging. Background technique [0002] Aging is a biological process in which organisms gradually degenerate over time at the cellular, tissue, organ, and individual levels, leading to the end of life. Plant senescence is a decline process at the organ level, often accompanied by the death of mesophyll cells and individuals, such as the senescence of annual plants such as rice, corn and soybean during the filling period of leaves. For perennials such as trees, senescence is mainly manifested in changes in leaf color in autumn. [0003] Plant senescence is not a passive and disorderly decline process, but essentially a programmed death process of mesophyll cells. Leaf is the main place for photosynthesis of plants. After a period of photosynthesis and accumulation of nutrients, mesophyll cells enter the senescence period. During s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84
Inventor 左建儒王青粘金沯李家洋钱前谢先芝陈凡
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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