Application of microorganisms in identification and/or differentiation of individuals of different races
A microbial and ethnic technology, applied in the field of biomedicine, can solve problems such as Acinetobacter genus that have not yet been seen
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Embodiment 1
[0078] 1. Materials and methods:
[0079] 1.1 Study population
[0080] A case-control study was designed according to the STROBE statement, and 128 Tibetan male soldiers who served in a certain Chinese army from a certain month to a certain month in a certain year were included as the experimental group, and 128 plain Han soldiers were matched according to the age of the Tibetan soldiers. for comparison. Strictly control the two groups of people to have the same mixed diet of Chinese troops, maintain the same training environment and training intensity at an altitude of 3500 meters, quit smoking and drinking, and last for 3 months. Soldiers with chronic inflammatory diseases, oral antibiotics, acute infections, and gastrointestinal disorders were excluded.
[0081] 1.2 Stool sample collection
[0082] Each research subject received a toilet feces collector after being included. After excreting the stool, put the stool into a stool collection tube and store it in a -20°C re...
Embodiment 2
[0106] Embodiment 2 verification experiment
[0107] Design a case-control study according to the STROBE statement, select 5 volunteers, and strictly control the 5 volunteers to have the same mixed diet of Chinese troops, maintain the same environment and exercise intensity at an altitude of 3500 meters, quit smoking and alcohol, and last for 3 months. Chronic inflammatory diseases, oral antibiotics, acute infections and gastrointestinal diseases were excluded in all 5 volunteers.
[0108] Experimental steps:
[0109] 1. Fecal sample collection;
[0110] 2. DNA extraction;
[0111] 3. PCR amplification, sample mixing and purification;
[0112] 4. Library construction and on-machine sequencing;
[0113] 5. Sequencing data processing;
[0114] 6. Operational taxon clustering and species annotation. The specific operations of steps 1-6 are as described in Example 1.
[0115] Experimental results
[0116] Among the 5 volunteers, 3 volunteers whose relative abundance value ...
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