Myoglobin time-resolved fluorescence immunochromatography testing kit

A time-resolved fluorescence and myoglobin technology, which is applied in the field of myoglobin time-resolved fluorescence immunochromatography assay kit and its preparation, can solve the problems of low detection sensitivity and measurement accuracy, and achieves the effect of convenient and fast operation.

Inactive Publication Date: 2019-06-21
TIANJIN MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the detection sensitivity and measurement accuracy are still not high

Method used

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  • Myoglobin time-resolved fluorescence immunochromatography testing kit
  • Myoglobin time-resolved fluorescence immunochromatography testing kit

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Embodiment Construction

[0048] Unless otherwise defined, the technical terms used in the following embodiments have the same meaning as commonly understood by those skilled in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are conventional biochemical reagents; the experimental methods, unless otherwise specified, are conventional methods.

[0049] All numerical designations, such as pH, temperature, time, concentration, including ranges, are approximate. It is to be understood that, although not always expressly stated, all numerical designations are preceded by the term "about". It is also to be understood that, although not always explicitly stated, the reagents described herein are merely exemplary and equivalents are known in the art.

[0050] The present invention will be described in detail below in conjunction with examples.

[0051] 1. Preparation of test card

[0052] 1) Preparation of detection area and quali...

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Abstract

The invention provides a myoglobin time-resolved fluorescence immunochromatography testing kit. The kit comprises a detection card and a fluorescent microsphere working fluid. The detection card comprises the detection card used for detecting myoglobin. The fluorescent microsphere working fluid contains a time-resolved fluorescence microsphere for marking a corresponding antibody. During a detection process, fluorescent microsphere compounds in a detection area and a quality control area are combined, fluorescence emitted under an effect of excitation light is collected and processed by a fluorescence immunoanalyzer, and then a detection result can be calculated. The kit has advantages that operation is simple; measurement is accurate; sensitivity is high; and specificity is high.

Description

technical field [0001] The invention belongs to the technical field of myocardial marker protein immune analysis, and in particular relates to a myoglobin time-resolved fluorescence immunochromatographic assay kit and a preparation method thereof. Background technique [0002] Myoglobin (MYO) is a heme protein similar to hemoglobin. Exist in muscle cells, the main function is to store oxygen, and it is very abundant in the myocardium. The molecular weight is 16700, and it is composed of a polypeptide chain containing 153 amino acid residues and a heme prosthetic group, and is in a compact spherical shape. Most of the hydrophobic side chains on the amino acid residues in the polypeptide chain are inside the molecule, and the hydrophilic side chains are mostly on the surface of the molecule, so its water solubility is better. [0003] Due to the small molecular weight of MYO, it is released into the blood circulation earlier after muscle cell damage, increases in 1-2 hours, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 汤乃军刘萍陈曦单安琪闫梦璠
Owner TIANJIN MEDICAL UNIV
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