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Application of syringaresinol in preparation of drugs for resisting rheumatoid arthritis

A syringaresin and rheumatoid technology, applied in the field of medicine, can solve problems such as poor action selectivity, damage, and induced infection

Inactive Publication Date: 2019-06-25
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, non-steroidal anti-inflammatory drugs (diclofenac, aspirin, etc.) and immunosuppressants (6-mercaptopurine, etc.) are mainly used for the treatment of rheumatoid arthritis, but these drugs all have obvious adverse reactions, such as the former damages the gastrointestinal tract tract mucosa leading to peptic ulcer, the latter has poor selectivity and is easy to induce infection, etc.

Method used

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  • Application of syringaresinol in preparation of drugs for resisting rheumatoid arthritis
  • Application of syringaresinol in preparation of drugs for resisting rheumatoid arthritis
  • Application of syringaresinol in preparation of drugs for resisting rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: The inhibitory effect of syringaresin on the proliferation of rheumatoid arthritis synoviocytes

[0022] Cells: human rheumatoid arthritis synoviocytes (RA-FLS);

[0023] Drugs: syringaresin;

[0024] Methods: 1. Resuscitation of RA-FLS

[0025] Quickly take out the RA-FLS cryopreservation tube from the liquid nitrogen irrigation, put it into a constant temperature water bath with a temperature of 37°C, and shake it continuously to speed up the thawing of the cells. After the thawing is complete, transfer the RA-FLS suspension in the cryovial to a 10mL sterile centrifuge tube in an ultra-clean bench, and centrifuge for 5min at a speed of 1000r / min. Pour off the supernatant, add 5mL of complete culture solution, pipette continuously to a uniform cell suspension, inoculate RA-FLS in a 25mL cell culture flask, and culture it in a cell culture incubator. The incubator condition is set to Temperature 37°C, CO 2 Concentration 5%. After 24 hours, the growth of ...

Embodiment 2

[0034] Example 2: The promoting effect of syringaresin on synovial cell apoptosis in rheumatoid arthritis

[0035] Cells: human rheumatoid arthritis synoviocytes (RA-FLS);

[0036] Drugs: syringaresin;

[0037] Methods: 1. Resuscitation of RA-FLS

[0038] Same as Example 1.

[0039] 2. Passage of RA-FLS

[0040] Same as Example 1.

[0041] 3. Determination of cell apoptosis by TUNEL method

[0042] The fragmentation of chromosomal DNA in apoptosis is a gradual and staged process. Chromosomal DNA is first degraded into large fragments of 50-300kb under the action of endogenous nucleolytic enzymes. Then about 30% of the chromosomal DNA is in Ca 2+ and Mg 2+ Under the action of dependent endonuclease, it is randomly cut between nucleosome units to form 180-200bp nucleosomal DNA polymers. The 3'-OH end of a series of DNA produced by DNA double-strand break or as long as there is a gap on one strand can be deoxyribonucleotide and fluorescein under the action of deoxyribonuc...

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Abstract

The invention discloses an application of syringaresinol in preparation of drugs for resisting rheumatoid arthritis. Syringaresinol is a ditetrahydrofuran lignan, and it is found for the first time that the syringaresinol can obviously inhibit the proliferation of rheumatoid arthritis synoviocytes and induce the apoptosis of rheumatoid arthritis synoviocytes. Therefore, the compound can be used for preparing the drugs for preventing and / or treating rheumatoid arthritis, and has higher clinical application value and development prospect.

Description

Technical field: [0001] The invention relates to the technical field of medicine, in particular to the use of syringaresin in the preparation of anti-rheumatoid arthritis drugs. Background technique: [0002] Rheumatoid arthritis (rheumatoid arthritis, RA) is a chronic systemic autoimmune disease mainly characterized by joint lesions. Its pathogenesis is still unclear. It is clinically believed that the pathogenesis of RA is mainly due to autoimmune dysfunction in patients. , leading to allergic reactions, degeneration of anti-inflammatory cells, deposition in joints and joint tissue deformity and disability. According to investigations, the incidence of RA worldwide is about 1-2%. The total number of RA patients in my country has exceeded 4 million, and the incidence is still on the rise. [0003] At present, non-steroidal anti-inflammatory drugs (diclofenac, aspirin, etc.) and immunosuppressants (6-mercaptopurine, etc.) are mainly used for the treatment of rheumatoid arth...

Claims

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Application Information

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IPC IPC(8): A61K31/34A61P19/02A61P29/00
Inventor 姚遥李娟杨丹李玮琦
Owner NINGXIA MEDICAL UNIV
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