Whole-blood microfluidic automatic cross-matching blood matching system

Abstract

The invention provides a whole-blood microfluidic automatic cross-matching blood matching system comprising a blood matching card, an incubating device connected with a sample outlet of the blood matching card, and a detecting device connected with a sample outlet of the incubating device. The incubating device includes a first mixed incubation zone and a second mixed incubation zone. The blood matching card includes a diluent rationing system, a blood supply rationing system, and a blood receiving rationing system. A sample outlet of a serum rationing region in the blood supply rationing system and a sample outlet of a red blood cell rationing region in the blood receiving rationing system are communicated with the first mixed incubation zone; a sample outlet of a red blood cell rationingregion in the blood supply rationing system and a sample outlet of a serum rationing region in the blood receiving rationing system are communicated with the second mixed incubation zone. According to the invention, automatic separation, rationing, mixing and detection of serum and red blood cells are realized; the operation is simple and convenient; and the integration and automation of cross-matching blood detection are realized. Besides, the invention also provides a blood matching method for the whole-blood micro-flow microfluidic automatic cross-matching blood matching system.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a whole blood microfluidic automatic cross-matching system and a blood-matching method. Background technique [0002] With the continuous development of clinical medicine, blood transfusion has become a necessary means of clinical rescue and treatment. However, in the clinical process of blood transfusion, adverse reactions occur from time to time, and these adverse reactions usually have serious consequences or even life-threatening. Therefore, it is an important prerequisite and guarantee for safe blood transfusion to conduct a cross-matching test before clinical blood transfusion to check the compatibility between the blood recipient's red blood cell antigen and the antibody in the serum and the blood donor. At present, the test methods commonly used for cross-matching in clinical medicine mainly include saline method, polybrene method, and antihuman glo...

AI Technical Summary

Problems solved by technology

The process of liquid preparation and sample addition is cumbersome, and it is easy to be confused during manual operation. For example, when adding samples, the red blood cell suspension does not fully contact with the serum sample or part of it enters the small column tube below, which may cause incomplete incubation and affect the test results. Put the card vertically on the incubator to incubate, and then put it on a special centrifuge for horizontal centrifugation. After centrifugation, return the reagent card to the vertical direction and use the camera system or the naked eye to interpret the results. The reagent card is on multiple operating platforms and in two directions. Frequent changes, complex operation

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