A method for reducing the contamination rate of endophytes in euonymus seeds and rapidly inducing embryoid bodies
A technology of embryoid body and contamination rate, which is applied in the biological field to achieve the effect of high seed germination rate and reduced endophyte contamination rate
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Embodiment 1
[0015] This implementation case provides a method for reducing the contamination rate of endophytic bacteria in euonymus seeds and rapidly inducing embryoid bodies, including the following steps:
[0016] 1) Seed collection: In mid-August, select the mature seeds of Euonymus euonymus (the outer green peel is cracked to reveal the red aril inside), pick them off together with the fruit pedicle, put them in a clean foam ice box and bring them back to the laboratory .
[0017] 2) Seed pre-treatment: In the afternoon, take out the euonymus seeds, remove the fruit pedicles, peel off the outer seed coat and aril, put them into a clean beaker, rinse them with running water, and then use 180 mg / L GA 3 The solution was soaked for 32 h, of which the first 16 h was placed in a 4°C refrigerator, and the last 16 h was placed in a 25-30°C incubator.
[0018] 3) Seed disinfection. Wash the pretreated seeds with running water for about 10 minutes, then soak them in detergent water for about...
Embodiment 2
[0024] This implementation case provides a method for reducing the contamination rate of endophytic bacteria in euonymus seeds and rapidly inducing embryoid bodies, including the following steps:
[0025] 1) Seed collection: At the beginning of September, select the mature seeds of Euonymus euonymus, pick them off together with the fruit stem, put them in a clean foam ice box and bring them back to the laboratory.
[0026] 2) Seed pre-treatment: In the afternoon, take out the euonymus seeds, remove the fruit pedicles, peel off the outer seed coat and aril, put them into a clean beaker, rinse them with running water, and then use 150 mg / L GA 3 The solution was soaked for 30 hours, of which the first 15 hours were placed in a 4°C refrigerator, and the last 15 hours were placed in a 25-30°C incubator.
[0027]3) Seed disinfection. Wash the pretreated seeds with running water for about 10 minutes, then soak them in detergent water for about 30 minutes, and then rinse them with ru...
Embodiment 3
[0031] This implementation case provides a method for reducing the contamination rate of endophytic bacteria in euonymus seeds and rapidly inducing embryoid bodies, including the following steps:
[0032] 1) Seed collection: In mid-September, select the mature seeds of Euonymus euonymus, pick them off together with the fruit stalks, put them in a clean foam ice box and bring them back to the laboratory.
[0033] 2) Seed pre-treatment: In the afternoon of the same day, take out the euonymus seeds, remove the fruit pedicles, peel off the outer seed coat and aril, put them into a clean beaker, rinse them with running water, and then use 200 mg / L GA 3 The solution was soaked for 36 h, of which the first 18 h was placed in a 4°C refrigerator, and the last 18 h was placed in a 25-30°C incubator.
[0034] 3) Seed disinfection. Wash the pretreated seeds with running water for about 10 minutes, then soak them in detergent water for about 30 minutes, and then rinse them with running wa...
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