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Microfluidic sorting chip and sorting system with the same

A sorting system and microfluidic technology, applied in material stimulation analysis, enzymology/microbiology devices, biochemical instruments, etc., can solve the problems of time-consuming and labor-consuming cells, difficult manipulation of optical tweezers, large diameter and mass of islet cells, etc. problem, achieving high spatial resolution and sensitivity

Inactive Publication Date: 2019-06-28
THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the diameter and mass of islet cells, the research object of this protocol, are relatively large, and optical tweezers are not easy to control, and the manipulation of cells by optical tweezers is time-consuming and labor-intensive.

Method used

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  • Microfluidic sorting chip and sorting system with the same
  • Microfluidic sorting chip and sorting system with the same
  • Microfluidic sorting chip and sorting system with the same

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Embodiment 3

[0084] A microfluidic sorting system, wherein the schematic diagram of cell sorting is as follows Figure 7 As shown, the cell sorting technology roadmap is as follows Figure 8 As shown, the logic of the control system is as follows Figure 9 As shown, the system specifically includes the following steps:

[0085] a1) Obtain cell spectral data:

[0086] In order to obtain the Raman spectrum of a certain type of pancreatic islet endocrine as a training sample, the applicant first collected the cell Raman spectrum data and then performed immunofluorescence to identify the cell type. The islet cells isolated from primary rats were grown adherently in the cell localization grid culture dish, the Raman spectrum data of all the cells grown in the coordinate system were collected and the spatial coordinates of the collected cells in the culture dish were recorded, and then the cultured Immunofluorescence staining was performed on all cells in the dish, because the grid can determ...

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Abstract

The invention provides a microfluidic sorting chip and a sorting system with the same, and belongs to the technical field of cell sorting. The microfluidic sorting chip comprises an air path control layer, a PDMS thin film layer, a fluid channel layer and a quartz glass substrate which are sequentially overlapped from top to bottom. The sorting system comprises a laser optical tweezer Raman spectrum system and the cell microfluidic sorting chip. The microfluidic sorting chip of a seven-way structure can realize the automatic guiding, detecting and sorting of detected cells, and the original-generation islet cell sorting efficiency and accuracy are improved; the image recognizing, Raman spectrum detecting and microfluidic technology and the like are adopted for the sorting system designed on the basis of the chip, the efficient, automatic and damage-free purification of the original-generation islet cells alpha, beta, delta, epsilon and pp can be realized, the original-generation pancreas islet can be studied on the level of single cells, and the important technological problems in the field are solved.

Description

technical field [0001] The invention belongs to the technical field of sorting primary pancreatic islet cells, and in particular relates to a microfluidic sorting chip and a sorting system thereof. Background technique [0002] The mammalian pancreas is divided into exocrine and endocrine divisions. Acini in the exocrine compartment secrete pancreatic juice, while endocrine compartment islets are scattered within the exocrine compartment. Pancreatic islets are composed of four different types of endocrine cells, which are alpha cells that produce glucagon, beta cells that produce insulin, delta cells that produce somatostatin, and pp cells that produce pancreatic polypeptide. [0003] At present, the cell models for studying islet cells in vitro mainly include islet cell tumor cell lines produced by α- and β-cell tumor clones and primary islet cells obtained from living animals. Compared with tumor cells, primary cells are closest to the biological properties of the body i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/00B01L3/00G01N21/65
Inventor 荣曦刘红
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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