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A strain of Brevibacterium flavum producing l-valine and its application

A technology of Brevibacterium flavum and valine, which is applied in the field of bioengineering and can solve problems such as the inability to produce L-valine

Active Publication Date: 2020-08-04
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these bacterial strains still can't meet the demand of industrial production L-valine

Method used

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  • A strain of Brevibacterium flavum producing l-valine and its application
  • A strain of Brevibacterium flavum producing l-valine and its application
  • A strain of Brevibacterium flavum producing l-valine and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Obtaining of Brevibacterium flavum FMME447

[0026] Screening liquid medium: valine 100g, glucose 5g, beef extract 5g, yeast powder 5g, peptone 10g, sodium chloride 5g, distilled water to 1L, pH 7.0-7.2, sterilized at 121°C for 30min.

[0027] Screening solid medium: valine 100g, glucose 5g, beef extract 5g, yeast powder 5g, peptone 10g, sodium chloride 5g, agar 20g, distilled water to 1L, pH7.0~7.2, sterilized at 121℃ for 30min .

[0028] A strain of Brevibacterium flavum was obtained by selection and breeding, which can grow normally on the screening liquid medium with a L-valine concentration of 100g / L and the screening solid medium with a L-valine concentration of 100g / L. Named Brevibacterium flavum FMME447.

[0029] Brevibacterium flavum FMME447, named Brevibacterium flavum FMME447 taxonomically, was deposited in the China Center for Type Culture Collection on January 17, 2019, with the deposit number CCTCCNO:M2019053, and the deposit address is Wuhan ...

Embodiment 2

[0030] Example 2: Fermentation of Brevibacterium flavum FMME447 to produce L-valine

[0031] Solid plate medium: glucose 5g, beef extract 5g, yeast extract 10g, peptone 5g, sodium chloride 5g, agar 20g, dilute to 1L with distilled water, pH 7.0, sterilize at 121°C for 30min.

[0032] Slant medium: 5g glucose, 5g beef extract, 10g yeast extract, 5g peptone, 5g sodium chloride, 20g agar, dilute to 1L with distilled water, pH 7.0, sterilize at 121°C for 30min.

[0033] Seed medium: glucose 20g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, corn steep liquor 20g, ammonium sulfate 3g, biotin 100μg, V B1 100μg, dilute to 1L with distilled water, adjust the pH to 7.0 with sodium hydroxide solution, and sterilize at 121°C for 30min.

[0034] Fermentation medium: initial glucose concentration 40g, corn steep liquor 10g, potassium dihydrogen phosphate 0.5g, magnesium sulfate heptahydrate 0.1g, V H 200 μg, V B1 200μg, dilute to 1L with distilled water, adjust the pH to ...

Embodiment 3

[0039] Example 3: Fermentation of Brevibacterium flavum FMME447 to produce L-valine

[0040] The same as the fermentation process of Example 2, the only difference is that the fermentation medium is different.

[0041] Fermentation medium: initial glucose concentration 50g, corn steep liquor 15g, potassium dihydrogen phosphate 1.0g, magnesium sulfate heptahydrate 0.3g, V H 200 μg, V B1 200μg, dilute to 1L with distilled water, adjust the pH to 7.0 with sodium hydroxide solution, and sterilize at 121°C for 30min.

[0042] After the fermentation, the fermented liquid was collected and centrifuged, and the supernatant was collected to measure the content of L-valine in the fermented liquid by high performance liquid chromatography to be 85.7 g / L.

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Abstract

The invention discloses a strain of brevibacterium flavum producing L-valine and application of the brevibacterium flavum, and belons to the technical field of bioengineering. The brevibacterium flavum is preserved in the China Center for Type Culture Collection on January 17, 2019 under the preservation number of CCTCC NO: M2019053, and the preservation address is Wuhan University, Wuhan, China.The brevibacterium flavum FMME447 exhibits the ability to accumulate high-level L-valine, and it is verified that the valine tolerance of the brevibacterium flavum reaches 100 g / L. The yield of the L-valine through the fermentation of the brevibacterium flavum reaches 80-90 g / L, the yield of glucose reaches up to about 25-30%, and the brevibacterium flavum has industrial practical value.

Description

technical field [0001] The invention relates to an L-valine-producing Brevibacterium flavum and its application, belonging to the technical field of bioengineering. Background technique [0002] L-valine is a branched-chain amino acid, which is one of the essential amino acids for the human body. It has a variety of physiological functions and is mainly used in the manufacture of food, flavoring agents, animal feed and cosmetics, as well as in the preparation of compound amino acid infusions, synthetic polypeptide drugs and food The role of antioxidants, etc., especially in hospital research and treatment, has been paid more and more attention. L-valine in the treatment of blood-brain barrier, hepatic coma, chronic liver cirrhosis and renal failure, dietary treatment of congenital metabolic defects, treatment of sepsis and postoperative diabetic patients, acceleration of surgical wound healing, nutrition of tumor patients Widely used in supportive care. With the continuous...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/08C12R1/13
Inventor 罗秋玲刘立明季立豪张权刘佳陈修来
Owner JIANGNAN UNIV
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