The Rapid Propagation Method of Emei Trough Glossus
A fast technology of quillia, which is applied in the field of plant propagation, can solve the problems of lack of quillia plants in Emei, threat of wild populations, and scarcity of quantity, etc.
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Embodiment 1
[0025] a. Acquisition of capsules: After near-field protection and years of growth adaptability research, select plants with strong adaptability and vigorous growth. During the flowering period, select plants from different populations for artificial pollination. The seed setting rate is 100%. Obtained For healthy capsules, mark the hybrid combination and pollination time when pollinating, and the capsules are picked when they are not cracked 385 days after pollination;
[0026] b. Treatment of capsules: pick the uncracked capsules of Emei groves, rinse with tap water for 8 minutes, put them on the ultra-clean workbench for standby, and treat them with alcohol with a mass concentration of 75% for 35 seconds and a mass concentration of 0.1% under sterile conditions. Sterilize with mercuric chloride solution for 18 minutes, rinse with sterile water for 6 times, and then use sterile filter paper to absorb the water on the surface of the capsule; In a Petri dish, mix the seeds eve...
Embodiment 2
[0032] a. Acquisition of capsules: After near-field protection and years of growth adaptability research, select plants with strong adaptability and vigorous growth. During the flowering period, select plants from different populations for artificial pollination. The seed setting rate is 100%. Obtained Healthy capsules; mark the hybrid combination and pollination time when pollinating, and the capsules are picked when they are not cracked 520 days after pollination.
[0033] b. Treatment of capsules: pick the uncracked capsules of Glossia emeiensis, wash them with tap water for 8 minutes, and put them on the ultra-clean workbench for later use. Under aseptic conditions, they were treated with 75% alcohol for 35 seconds, sterilized with 0.1% mercuric chloride solution for 18 minutes, rinsed with sterile water for 6 times, and then blotted dry the water on the surface of the capsule with sterile filter paper. Cut open the sterile capsule with a sterilized knife;
[0034] c Indu...
Embodiment 3
[0039] a. Acquisition of capsules: After near-field protection and years of growth adaptability research, select plants with strong adaptability and vigorous growth. During the flowering period, select plants from different populations for artificial pollination. The seed setting rate is 100%. Obtained For healthy capsules, mark the hybrid combination and pollination time when pollinating, and the capsules are picked when they are not cracked 500 days after pollination;
[0040] b. Treatment of capsules: pick the uncracked capsules of Eucalyptus emei, rinse them with tap water for 5 minutes, put them on the ultra-clean workbench for later use, and treat them with alcohol with a mass concentration of 75% for 30 seconds and a mass concentration of 0.1% under sterile conditions. Sterilize with mercuric chloride solution for 15 minutes, and rinse with sterile water for 4 times respectively, and then use sterile filter paper to blot the water on the surface of the capsule; In a Pet...
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