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A kind of biocontrol bacterium of mulberry tree disease and its application

A technology for mulberry and disease, which is applied in the field of bio-control bacteria for mulberry bacterial diseases, can solve the problems of soil environment deterioration, difficult control, and enhanced drug resistance of pathogens, and achieve good application prospects and avoid the effect of soil environment deterioration

Active Publication Date: 2021-08-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most of the mulberry diseases are soil-borne diseases, which are difficult to prevent and control. At present, chemical pesticides are mainly used to alleviate the disease. Chemical pesticides have the advantage of quick effect and can quickly alleviate the disease. However, large-scale use will deteriorate the soil environment and cause pathogenic Enhanced drug resistance cannot fundamentally solve the problem, so it is urgent to find a more effective, long-term and safe prevention method

Method used

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  • A kind of biocontrol bacterium of mulberry tree disease and its application
  • A kind of biocontrol bacterium of mulberry tree disease and its application
  • A kind of biocontrol bacterium of mulberry tree disease and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The separation and purification of embodiment 1 root endophyte

[0043] 1. Experimental method

[0044] 1. Isolation and purification of root endophytes

[0045] Take the freshly collected mulberry root tissue and rinse it with tap water, cut into about 3-4 cm root segments and place them in a funnel with a switch (the funnel has a filter screen), and then add 75 % alcohol, soaked for about 15s, then drained the alcohol, treated with mercuric chloride for 5 minutes in the same way, and then rinsed with sterile water for 3 to 4 times, took out the root segment and put it on the workbench to air dry, then cut the root segment with sterile tweezers. The cortex is torn off to expose the xylem, and then the longitudinal strips of the xylem are scraped off with a sterile scalpel and placed in a sterile mortar. After adding about 5mL of sterile water, grind it thoroughly. Use a pipette to take 1mL of the grinding solution and add 9mL of it. In sterile water, release 5 times ...

Embodiment 2

[0057] Identification of embodiment 2 antagonistic bacteria

[0058] The culture characteristics of the colony plate, Gram staining and various physiological and biochemical index tests are important basis for determining the classification status of bacterial strains. Because rRNA gene sequence is highly conserved, it can be used as an important reference standard for evolutionary analysis.

[0059] 1. Experimental method

[0060] The screened antagonistic strain (YD-001) was inoculated in beef extract peptone medium, cultured at 28°C for 2 days, and the colony morphology, growth status, soluble pigment production, etc. were observed and recorded. Gram staining, morphological observation, contact enzymes, aerobicity and other related physical and chemical parameters were determined according to the "Common Bacterial System Identification Manual" and "Berger Bacterial Identification Manual".

[0061] The entire genome of the antagonistic bacteria (YD001) was sequenced, and t...

Embodiment 3

[0070] Pot control of embodiment 3 antagonistic bacteria YD-001

[0071] 1. Experimental method

[0072] The soil used in the pot experiment was humus soil, and the mulberry seed variety was selected as the susceptible variety "Guisang 12". The pathogens of mulberry wilt were isolated and preserved in this experiment, including Enterobacter cloacae, Pantoea, Raueria morulus, and Klebsiella. The mulberry seedlings are 50d seedlings. Experimental group, for testing mulberry seedlings with 10 6 The cfu / mL antagonistic bacteria YD-001 strain suspension soaked the roots for 1 min, and then poured the bacterial suspension once every other week for a total of 2 times. After 20 days, the wounded root method was used to inoculate the roots for 10 days. 8 cfu / mL pathogenic bacteria suspension 10ml. The blank control group was only inoculated with pathogenic bacteria. 30 mulberry seedlings per treatment. After 20 days, investigate and record the incidence of mulberry plants, and ca...

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Abstract

The invention discloses a biocontrol fungus for mulberry diseases and application thereof. The invention provides a Pseudomonas aeruginosa strain (Pseudomonas aeruginosa) for preventing and treating mulberry wilt. The strain was preserved in the Guangdong Microbial Culture Collection Center on March 19, 2019, and the preservation number is GDMCC No: 60613. The present invention obtains for the first time a Pseudomonas aeruginosa strain that has good antagonism / control effects on various pathogens of mulberry wilt. Using the bacterial strain provided by the invention or its fermented liquid to carry out biological means to prevent and control mulberry bacterial wilt is safe and effective, and can avoid various problems such as deterioration of the soil environment caused by chemical pesticides and enhanced drug resistance of pathogens. The biological control has laid the foundation, has a good application prospect, and is worthy of vigorous promotion.

Description

technical field [0001] The invention relates to the technical field of plant disease prevention and control, in particular to a biocontrol fungus for bacterial diseases of mulberry trees and its application. Background technique [0002] Mulberry tree disease refers to the phenomenon of poor growth and development, reduced yield and poor quality of mulberry leaves due to the infection of pathogenic microorganisms. Due to the existence of the above-mentioned diseases, the quality and yield of mulberry leaves have been seriously affected, and the development of mulberry planting industry has been hindered. [0003] Mulberry bacterial wilt (Mulberry bacterial wilt) has another name called mulberry wilt, and mulberry bacterial wilt (Mulberry bacterial wilt) and mulberry blight (Mulberry bacterial blight) are the three major mulberry bacterial diseases in my country. The disease has a rapid onset and rapid spread, seriously affecting the yield and quality of mulberry, and causin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/27A01P1/00C12R1/385
CPCA01N63/00C12R2001/385C12N1/205A01N63/10
Inventor 刘吉平王继承罗龙辉
Owner SOUTH CHINA AGRI UNIV
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