Procambarus clarkii C-type lectin gLecB gene and encoded gLecB protein thereof

A technology of Crayfish and gene encoding, applied in the field of genetic engineering, can solve the problems of lack of in-depth analysis of shrimp, lack of cell line support, etc.

Inactive Publication Date: 2019-07-23
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still a lack of in-depth analysis of the genome information of shrimp, and the basic research of shrimp and crab lacks the support of cell lines

Method used

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  • Procambarus clarkii C-type lectin gLecB gene and encoded gLecB protein thereof
  • Procambarus clarkii C-type lectin gLecB gene and encoded gLecB protein thereof
  • Procambarus clarkii C-type lectin gLecB gene and encoded gLecB protein thereof

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Experimental program
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Embodiment

[0081] The method for constructing the expression vector provided in the embodiments of the present invention includes:

[0082] 1. RNA extraction

[0083] Inhale 0.5ml of anticoagulant with a 2ml disposable syringe, after drawing blood, mix immediately, collect into 1.5ml RNA freeEP tube, centrifuge at 3000r, 10min, 4°C, remove supernatant and add 1ml RNAiso Plus to blow evenly; other tissues The sample was ground completely with a grinder, and 1 mL RNAiso Plus was added to the grinder in advance. Then collected into 1.5mL RNA free EP tube, the whole operation on ice. Add 400 μl of chloroform to the collected samples, mix gently, and let stand on ice for 5 minutes. Then centrifuge at 4°C for 10 000r15min, suck 400μl supernatant into a new 1.5ml RNA free EP tube, add 600μl isopropanol, mix gently and place in -20°C refrigerator for 2h to allow RNA to settle; then 10 000r, Centrifuge at 4°C for 10min, discard the supernatant; add 1ml of 75% ethanol, blow and wash the precipi...

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Abstract

The present invention belongs to the technical field of genetic engineering and discloses a procambarus clarkii C-type lectin gLecB gene and an encoded gLecB protein thereof. A sequence of the procambarus clarkii C-type lectin gLecB gene is shown in SEQ ID NO:1; an amino acid sequence of the gLecB protein is shown in SEQ ID NO:2; and an expression vector is pGEX-5X-1-B-Lectin. The new C-type lectin gLecB is isolated from procambarus clarkii and has an antiviral effect.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a C-type lectin gLecB gene of Procambarus clarkii and the encoded gLecB protein. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Due to the lack of a specific immune mechanism based on "antibody-antigen recognition", invertebrates such as shrimp and crab rely only on pattern recognition receptors (PRRs) encoded by germline genes and related immune regulatory systems to resist pathogenic invasion. Immune recognition is the first step in the immune response. Invertebrates mainly rely on pattern recognition receptors (PRRs) to recognize invading pathogens, that is, the aforementioned pathogen-related molecular patterns PAMPs can be recognized by pattern recognition as ligands. Body PRRs identification. On the one hand, PRRs can directly kill and clear pathogenic bacteria, on the othe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/22C12N15/12C12N15/70C12N15/66A61P31/12
CPCA61P31/12C07K14/43509C12N15/66C12N15/70
Inventor 兰江风顾泽茂张英豪李通曹晓彤孙萌
Owner HUAZHONG AGRI UNIV
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