Acoustic tweezers
An electro-acoustic and surface acoustic wave technology, applied in optics, optical components, scientific instruments, etc., can solve problems such as the impermissibility of acoustic standing wave methods
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example 5
[0279] Example 5: Cell manipulation
[0280] Such as Figure 22 As shown, manipulations of cells and droplets are performed with a microscope, such as to generate homogeneous or heterogeneous cellular networks, such as stem cell niches, made of stem cells with similar physical properties.
[0281] Droplets are the basis of droplet-based microfluidics for use in the field of single-cell biology. The electroacoustic device of the present invention allows the in-depth study of rare events, currently a major problem in cancer and drug resistance research, by sampling them in a large number of experiments.
[0282] In this view, the central region of the transducer is placed beneath a set of particles to be manipulated by displacement provided by the micromanipulator. When the particle is in the center of the transducer's central area, turn on the power to generate the vortex SAW so that the particle is attracted by the dark spot of the SAW. The operating frequency is 30MHz and ...
example 6
[0287] Example 6: Cell Morphing
[0288] Electroacoustic devices have also been implemented to exert forces on biological cells and particles.
[0289] It is now understood that forces and pressures on cells can determine their fate. Somatic cells are stress-adapted and may become rigid, and stem cell differentiation may be affected by external mechanical stress. However, the method is limited to applying stress to the cells.
[0290] A liquid medium containing antibody-coated microspheres and cell membranes is placed below the object to be manipulated by the displacement provided by the micromanipulator. Suitable transducers are motorized to trap the antibody-coated microspheres on top of the center of the transducer. Simultaneously with the application of electrical energy, the support is displaced such that the cell membrane is in contact with and deformed by the antibody-coated microspheres.
example 7
[0291] Example 7: Steady current and vorticity
[0292] A vortex SAW is generated to create a stable swirl in a microchannel, which is useful for non-contact mixing, or for applying hydrodynamic stress or for moving particles with a size smaller than λ / 10.
[0293] Flow velocity is proportional to the acoustic power in the medium and increases with any one of the square of the wave frequency, the vortex order, and the square of the channel height.
[0294] A chamber with grooves defining microchannels is placed on the support, the grooves being positioned perpendicular to the center of the transducer. A liquid medium with a set of particles is placed in the microchannel.
[0295] The depth of the groove is preferably greater than λ, λ being the wavelength of the vortex SAW. Powering the transducer results in the flow observed in the microchannel in the form of a cyclone formed in the liquid medium with its eye at the center of the radiating vortex SAW. To facilitate flow, t...
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