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Colonic organoids and methods of making and using same

An organoid, colon technology, applied in the field of colon organoids and their preparation and use, can solve the problems of lack of robust understanding of posterior bowel development and backward efforts in large intestine organoids

Pending Publication Date: 2019-07-26
CHILDRENS HOSPITAL MEDICAL CENT CINCINNATI
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Problems solved by technology

[0003] While the generation of gastric and small intestine organoids from pluripotent stem cells (PSCs) has revolutionized the study of human gastrointestinal (GI) development and disease, efforts to generate large intestine organoids have lagged behind, in part due to a lack of insight into hindgut tube development. robust understanding

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  • Colonic organoids and methods of making and using same
  • Colonic organoids and methods of making and using same
  • Colonic organoids and methods of making and using same

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[0082] The following non-limiting examples are provided to further illustrate the aspects of the invention disclosed herein. Those skilled in the art should understand that the technical representatives disclosed in the following examples have found methods that work well in the practice of the present invention, and therefore can be considered as examples of modes constituting their practice. However, according to the present disclosure, those skilled in the art should understand that without departing from the spirit and scope of the present invention, many changes can be made to the specific aspects disclosed and still obtain the same or similar results.

[0083] The epithelium of the gastrointestinal tract is derived from the definitive endoderm, which is one of the main germ layers established during gastrulation. The process of bowel morphogenesis transforms the definitive endoderm into a primitive bowel with foregut, midgut and hindgut. The midgut produces the small proxi...

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Abstract

Disclosed herein are methods for the in vitro differentiation of a precursor cell into definitive endoderm, which may further be differentiated into a human colonic organoid (HCO), via modulation of signaling pathways. Further disclosed are HCOs and methods of using HCOs, which may be used, for example, for the HCOs may be used to determine the efficacy and / or toxicity of a potential therapeutic agent for a disease selected from colitis, colon cancer, polyposis syndromes, and / or irritable bowel syndrome.

Description

[0001] Cross reference of related applications [0002] This application claims the benefit of U.S. Provisional Application Serial No. 62 / 429,948 filed on December 5, 2016, the entire content of which is incorporated herein by reference for all purposes. Background technique [0003] Although the production of gastric and small intestine organoids from pluripotent stem cells (PSC) has revolutionized research on human gastrointestinal (GI) development and disease, efforts to produce large intestine organoids have fallen behind, partly due to the lack of support for the development of the hindgut. Robust understanding. Summary of the invention [0004] Disclosed herein is a method for in vitro differentiation of precursor cells into definitive endoderm by modulating signal transduction pathways, which can be further differentiated into human colon organoids (HCO). HCO and methods of using HCO are further disclosed, which can be used. For example, HCO can be used to determine the effi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/485C07K14/50C07K14/51C12N5/00
CPCC07K14/50C07K14/51C07K14/485C12N5/0679C12N2501/155C12N2501/41C12N2506/02C12N2506/45C12N2501/415C12N2501/16C12N2501/119C12N2513/00C12N2533/90C12N2501/11C12N5/0697C12N2503/02G01N33/5088C12N2501/727C12N2501/113C12N2501/115C12N2501/117
Inventor J·M·威尔斯J·O·穆尼拉
Owner CHILDRENS HOSPITAL MEDICAL CENT CINCINNATI
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