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Primer set and detection method for detecting candida albicans in female vaginal secretions

A technology for Candida albicans and vaginal secretions, which is applied to a rapid quantitative detection kit for detecting Candida albicans in female vaginal secretions, the detection of Candida albicans in female vaginal secretions, and the rapid quantification of Candida albicans DNA in female vaginal secretions In the field of primer sets for detection, it can solve the problems of time-consuming fungi, biohazard risks, and the ability of fungal strains to grow, and achieve the effect of fast time.

Inactive Publication Date: 2019-08-06
SHANGHAI CITY JIADING DISTRICT CENT HOSPITAL
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, current detection and identification of fungi by culture-based methods is time-consuming, poses a biohazard risk, and the ability to support the growth of fungal strains is dependent on the culture medium

Method used

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  • Primer set and detection method for detecting candida albicans in female vaginal secretions
  • Primer set and detection method for detecting candida albicans in female vaginal secretions
  • Primer set and detection method for detecting candida albicans in female vaginal secretions

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Embodiment Construction

[0022] The present invention is further described below.

[0023] Operation steps of the present invention are:

[0024] 1. Extract DNA;

[0025] Using the GeneJET DNA / RNA Purification Kit (Thermo Fisher Scientific, USA), DNA was extracted from female vaginal secretions according to the instructions.

[0026] 2. Primer sequence:

[0027]

[0028] 3. Use the DNA extracted in step 1 as a template to carry out the LAMP amplification reaction, and the total reaction volume of the LAMP detection reaction system is 25 μl.

[0029] 4. The LAMP detection reaction system is:

[0030] dNTPs (1.4mM), Tris-HCl (pH = 8.8, 20mM), (NH 4 ) 2 SO 4 (10mM), KCl(10mM), MgSO 4 (8mM), betaine (0.8M), 0.1% Tween 20, Bst DNA polymerase (8U), AMV reverse transcriptase (0.5U), fluorescent dye (SYBR green I 1μl), Candida albicans RNA (2μl). Amplify at 63 degrees Celsius for 1 hour, and place the reaction tube in a water bath at 80 degrees Celsius for 5 minutes to terminate the reaction. Obse...

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Abstract

The present invention discloses a primer set for detecting candida albicans in female vaginal secretions. The primer set comprises an external primer pair F3 / B3 and an internal primer pair FIP / BIP. The present invention also relates to a method for detecting the candida albicans in the female vaginal secretions by using the primer set and also relates to a rapid quantitative detection kit for detecting the e candida albicans in the female vaginal secretions by using the primer set. The primer set has an advantage of providing results in a short period of time, much faster than time required for detecting and identifying fungi based on a culture method.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and in particular relates to a primer set for rapid quantitative detection of Candida albicans DNA in female vaginal secretions. [0002] The present invention also relates to a detection method for detecting Candida albicans in female vaginal secretions by using the above primer set. [0003] The present invention also relates to a rapid quantitative detection kit for detecting Candida albicans in female vaginal secretions by using the above primer set. Background technique [0004] Monilia albican (or canidia Albicans) is a fungus whose growth in the environment may cause allergies, mold toxicity, or severe mycoses. Fungal spores in the ambient air have been associated with acute asthma and infections. However, current detection and identification of fungi by culture-based methods is time-consuming, poses a biohazard risk, and the ability to support the growth of fungal strains is depe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851C12Q1/689C12Q1/06C12N15/11
CPCC12Q1/6851C12Q1/689C12Q2531/119C12Q2563/107
Inventor 孙杰沈丽娟徐炜新于修文时建英姚娟史俊峰易清清
Owner SHANGHAI CITY JIADING DISTRICT CENT HOSPITAL
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