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Method for determining cystine content in compound amino acid injection (5%)

A determination method and amino acid technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that cystine content cannot be detected, and achieve the effect of simple method and low cost

Active Publication Date: 2019-08-06
SHANDONG POLYTECHNIC COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The method for measuring cystine in the compound 18AA-II amino acid injection (5%) provided by the present invention adopts a commonly used amino acid analyzer for detection. The problem of undetectable cystine content in

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  • Method for determining cystine content in compound amino acid injection (5%)
  • Method for determining cystine content in compound amino acid injection (5%)
  • Method for determining cystine content in compound amino acid injection (5%)

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Experimental program
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Embodiment 1

[0008] Test sample: Compound amino acid injection 18AA-II 250ml: 12.5g (total amino acid) Manufacturer: Fresenius Kabi Huarui Pharmaceutical Co., Ltd.

[0009] Inspection equipment: Hitachi L-8080 amino acid analyzer

[0010] Column: Ion Exchange Column #2622SC-PH 4.6*60mm

[0011] Mobile phase: B1, B2, B3, B4 are acidic buffer solutions with different pH, B5 is ultrapure water, and B6 is lye, all of which are dedicated to equipment.

[0012] Reaction phase: R1 is ninhydrin solution, R2 is ninhydrin buffer solution, R3 is 5% ethanol water for cleaning.

[0013] Chromatographic conditions:

[0014] High temperature reactor temperature: 130°C; flow rate of ninhydrin: 0.25ml / min; column temperature for amino acid analysis: 57°C, collection time: 53min, flow rate: 0.4ml / min, detection wavelengths are 440nm and 570nm

[0015] time B1% B2% B3% B4% B5% B6% R1% R2% R3% 0.0 100 2.5 100 2.6 100 4.5 ...

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Abstract

The invention provides a method for determining a cystine content in a compound 18AA-II amino acid injection (5%). The method is characterized by performing heat treatment for 1.5-2 hours before sample introduction to eliminate interference of sodium metabisulfite on cystine detection. The detection method provided by the invention is simple and low in cost, and solves the problem that the cystinecontent in the compound 18AA-II amino acid injection (5%) cannot be detected.

Description

technical field [0001] The invention relates to the field of medicines, in particular to a method for determining cystine in compound 18AA-II amino acid injection (5%). Background technique [0002] Amino acid drugs are suitable for the treatment of amino acid deficiency in the body under various conditions. Compound 18AA-II Amino Acid Injection (5%) contains 18 kinds of amino acids. Due to the large number of main components and the small difference in solubility, it is impossible to obtain a qualified resolution by conventional chromatographic column polar elution. The current content detection method Multi-purpose ion-exchange chromatography, according to the degree of affinity between various amino acids and cation-exchange chromatographic columns, use mobile phases of different pH to elute according to the gradient, and the amino acid components eluted successively are reacted with ninhydrin to develop color Ultraviolet absorption detection was carried out, and the com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/12
CPCG01N30/02G01N30/12
Inventor 刘登
Owner SHANDONG POLYTECHNIC COLLEGE