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Method for non-destructive and rapid identification of all silty and waxy corn germplasm by using optical illuminometer

A technology of waxy corn and illuminance meter, which is applied in photometry, optical radiation measurement, and material analysis through optical means, etc. It can solve the damage of corn seeds, distinguish between full powdery, waxy waxy corn, and corn seeds that cannot be regenerated Use follow-up planting and other issues to achieve the effect of low equipment requirements and rapid and accurate judgment results

Inactive Publication Date: 2019-08-13
INST OF TOBACCO ANHUI ACADEMY OF AGRI SCI
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Problems solved by technology

In addition, previous breeders did not distinguish between powdery and waxy waxy maize from the starch structure, ignoring the advantages of full powdery waxy maize in quality and taste, which affected the breeding and application of all powdery waxy maize varieties
[0003] Conventional identification methods have caused great damage to corn seeds. The cut corn seeds cannot be used for subsequent planting, and the corn seeds are small. When a large number of eyelids need to be detected, it takes a lot of time, manpower and material resources, and When observing the powder quality with the naked eye, since there is no quantitative index, the judgment accuracy will also be reduced

Method used

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  • Method for non-destructive and rapid identification of all silty and waxy corn germplasm by using optical illuminometer
  • Method for non-destructive and rapid identification of all silty and waxy corn germplasm by using optical illuminometer
  • Method for non-destructive and rapid identification of all silty and waxy corn germplasm by using optical illuminometer

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Embodiment 1

[0018] 1. Materials

[0019] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0020] The maize germplasms involved in this example (inbred lines 7015, 7058, 7123, 7156) were all obtained from the maize germplasm bank of the Tobacco Research Institute of Anhui Academy of Agricultural Sciences.

[0021] 2. Method

[0022] (1). The high-generation stable genetic maize inbred lines 7015(A), 7058(B), 7123(C) and 7156(D) to be tested were used for identification, among which, the color of 7015(A) and 7058(B) similar( figure 1 A, B), are white waxy maize germplasm; 7123(C) and 7156(D) are similar in color ( figure 1 C, D), for yellow waxy maize germplasm.

[0023] (2).Using an optical illuminance meter to measure the light transmission intensity of the seed endosperm

[0024] Step 1. Place the seed ...

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Abstract

The invention provides a method for non-destructive and rapid identification of all silty and waxy corn germplasm by using an optical illuminometer, and belongs to the technical field of corn germplasm identification methods. The method comprises the steps of: placing a seed endosperm upward on an optical measuring point of the illuminometer, irradiating the seed endosperm with a miniature fixed white light source from top leaning against the pericarp, recording light intensity reading on the illuminometer, judging whether the seed is an all silty seed according to the light intensity of the light source passing through the seed endosperm, if the light intensity of the light source passing through the seed endosperm is less than 5 lux, indicating that the seed is an all silty waxy corn, and if the light intensity of the light source passing through the seed endosperm is greater than 100 lux, indicating that the seed is waxy corn. According to the method, the all silty and waxy corn germplasm is identified according to the optical properties of different structural starches, the cultivation and verification of all silty and waxy corn selfing lines can be assisted by the method, as well as the breeding of all silty and waxy corn new species, so that the application prospect is very good.

Description

technical field [0001] The invention relates to the technical field of identification methods of maize germplasm, in particular to a method for non-destructive and rapid identification of whole farina and waxy maize germplasm by using an optical illuminance meter. Background technique [0002] Waxy corn can be divided into three types: white waxy, yellow waxy, and colorful waxy according to the difference in color. According to its internal starch structure, it can be divided into full powdery and waxy waxy corn. Most of the previous research focused on the taste of corn. Through Tasting is used to distinguish the quality of waxy corn, while ignoring the influence of the internal starch structure on the quality and taste of waxy corn. Compared with waxy waxy corn, full powdery waxy corn has a moister and sweeter taste, more easily absorbed starch, and a moderate ratio of crude fiber to nutrients, and is more suitable for processing and eating such as steaming and cooking. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/17G01J1/00
CPCG01J1/00G01N21/17
Inventor 李廷春董庆杨华应刘桂虎周应兵陈洪俭李成
Owner INST OF TOBACCO ANHUI ACADEMY OF AGRI SCI
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