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Method for adsorbing tuberculosis bacilli by macroporous resin

A technology based on macroporous resin and Mycobacterium tuberculosis, applied in the direction of microorganism-based methods, biochemical equipment and methods, specific-purpose bioreactors/fermenters, etc., can solve problems such as inconvenient handling and affecting the positive rate, and achieve improved Accuracy, strong adsorption effect

Active Publication Date: 2019-08-16
YICHUN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a method for absorbing Mycobacterium tuberculosis by using macroporous resin, which solves the problem that currently the method of collecting Mycobacterium tuberculosis in clinical specimens is mainly collected by means of centrifugation, which is very difficult to handle. Inconvenient, and there are many sediments in urine and other specimens, which often affect the positive rate of detection

Method used

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  • Method for adsorbing tuberculosis bacilli by macroporous resin
  • Method for adsorbing tuberculosis bacilli by macroporous resin

Examples

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Effect test

Embodiment 1

[0024] A method for absorbing Mycobacterium tuberculosis by using macroporous resin, comprising the steps of: (1) using a pretreatment device to process the macroporous resin, removing dust on the surface of the macroporous resin, so that the surface of the macroporous resin is clean and dry, (2) Add urine and pleural effusion into the beaker, then add 2 g of the macroporous resin D101 treated in step (1) to the beaker, mix well and let it stand for adsorption for 24 hours; The water port releases the adsorbed bacterial solution. After fully releasing the bacterial solution, close the water outlet of the beaker, add the eluent into the beaker, mix the macroporous resin and the eluent fully, release the eluent, and The content of Mycobacterium tuberculosis was detected in the eluate.

Embodiment 2

[0026] A method for absorbing Mycobacterium tuberculosis by using macroporous resin, comprising the steps of: (1) using a pretreatment device to process the macroporous resin, removing dust on the surface of the macroporous resin, so that the surface of the macroporous resin is clean and dry, (2) Add urine and pleural effusion into the beaker, then add 2g of the macroporous resin AB-8 treated in step (1) to the beaker, mix thoroughly and let it stand for adsorption for 24h; (3) After fully absorbing, use the Let go of the adsorbed bacterial solution at the water outlet of the beaker, after fully releasing the bacterial solution, close the water outlet of the beaker, add the eluent into the beaker, mix the macroporous resin and the eluent fully, and release the eluent , to detect the content of Mycobacterium tuberculosis in the eluate.

Embodiment 3

[0028] A method for absorbing Mycobacterium tuberculosis by using macroporous resin, comprising the steps of: (1) using a pretreatment device to process the macroporous resin, removing dust on the surface of the macroporous resin, so that the surface of the macroporous resin is clean and dry, (2) Add urine and pleural effusion into the beaker, then add 2g of the macroporous resin HPD100 treated in step (1) to the beaker, mix well and let it stand for adsorption for 24h; The water port releases the adsorbed bacterial solution. After fully releasing the bacterial solution, close the water outlet of the beaker, add the eluent into the beaker, mix the macroporous resin and the eluent fully, release the eluent, and The content of Mycobacterium tuberculosis was detected in the eluate.

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Abstract

The invention discloses a method for adsorbing tuberculosis bacilli by macroporous resin, wherein the method comprises the following steps: (1) treating macroporous resin with a pretreatment device, removing dust on the surface of the macroporous resin, and making the surface of macroporous resin clean and dry; (2) adding 10 ml of a bacterial liquid containing tuberculosis bacilli such as urine and hydrothorax into a beaker, then adding the macroporous resin treated in the step (1) to the beaker, fully mixing evenly and carrying out static adsorption for 24 h; and (3) after full adsorption, draining the adsorbed bacterial liquid with a water outlet of the beaker, fully draining the bacterial liquid, then closing the water outlet of the beaker, adding an eluate to the beaker, mixing the macroporous resin with the eluate evenly and fully, draining the eluate, and detecting the content of tuberculosis bacilli in the eluate. A centrifugal precipitation method in the prior art is preventedfrom using, the macroporous resin is used for enriching tuberculosis bacilli in clinical specimens such as hydrothorax and urine, and the accuracy of clinical detection is further improved.

Description

technical field [0001] The invention relates to the technical field of Mycobacterium tuberculosis concentration, in particular to a method for adsorbing Mycobacterium tuberculosis by using a macroporous resin. Background technique [0002] In the clinical diagnosis of tuberculosis, enriching and concentrating Mycobacterium tuberculosis from specimens is a basic and primary task, which is related to the accuracy of detection. At present, the concentrated bacteria collection method, that is, centrifugal precipitation, is mainly used to collect Mycobacterium tuberculosis in clinical specimens. This method is very inconvenient to handle, and there are many sediments in urine and other specimens, which often affect the positive rate of detection. Macroporous resin is a high-efficiency adsorbent, which is often used for the enrichment of drugs, so the present invention applies macroporous resin to pleural effusion, urine and other specimens to improve the accuracy of clinical dete...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12M1/00C12M1/04C12R1/32
CPCC12N1/20C12N1/04C12M23/02C12M29/06Y02A50/30
Inventor 周智兴邓琴
Owner YICHUN UNIVERSITY
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