Gastrointestinal malignant tumor marker and application thereof

Abstract

The invention relates to the field of a biomedicine, in particular to a gastrointestinal tumor marker and an application thereof. The marker is WNT4. The gastrointestinal malignant tumor is human gastrointestinal malignant tumor tissue, including gastric cancer and colorectal cancer. The marker is used for preparing a reagent for early diagnosis of gastrointestinal malignant tumor, a fluorescencequantitative PCR primer pair of WNT4 is used, a cDNA of a sample to-be-tested is used as a template for performing a quantitative PCR reaction, and a CT value of WNT4 amplification is measured, and the CT value of an internal reference gene beta-actin is used for standardization correction to identify the sample to-be-tested. Through a reliable fluorescent quantitative PCR method, the expression level of WNT4 in the gastrointestinal malignant tumor tissue is significantly reduced through screening. The method has high sensitivity and strong specificity, and the marker can be used for early diagnosis of gastrointestinal malignant tumors.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a gastrointestinal cancer marker and its application. Background technique [0002] With the development of society and economy and the change of dietary structure, the incidence of malignant tumors of gastrointestinal tract is increasing year by year. Gastrointestinal malignancies include gastric cancer and colorectal cancer. According to the latest statistics in 2018, gastric cancer and colorectal cancer rank third and fourth in the incidence and mortality of cancer in my country, posing a serious threat to people's lives and health. The gastrointestinal tract is the most important organ for digestion and absorption of nutrients in the human body, and the occurrence of malignant tumors in the gastrointestinal tract has a similar pathophysiological basis. Although a series of progress has been made in the mechanism of its occurrence, the exact etiology is still not completely clear....

AI Technical Summary

Problems solved by technology

However, the onset of gastrointestinal malignant tumors is hidden, and there are no obvious symptoms during the incubation period. When obvious symptoms appear, most of them have progressed to the middle and late stages, and the treatment is difficult. This also leads to the 5-year survival rate of patients with gastric cancer and colorectal cancer in my country is lower than the global level. one of the important reasons

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